SGER: A Rapid Method for Simultaneously Quantifying and Identifying Marine Microbes

9900301，提出了阿扎马方法，如果成功，将同时对环境样本中存在的大多数微生物进行量化和鉴定。这种方法是研究海洋盆地尺度上海洋微生物分布和多样性的先决条件。研究方法是基于这样一个事实，即七个碱基对的DNA标签，如果足够分散，可以区分数千个微生物物种。为了有用，这个标签区域必须总是位于微生物的基因组中，并且它必须与一个保守的DNA区域相关联，该保守DNA区域可以用于分离RNaseP基因座中的标签A标签区域，这是一种普遍存在的非酶，符合Bemg的标准，高度分散，位于保守区旁边。幸运的是，RNaseP基因座也总是以每个基因组一个拷贝的形式被发现。因此，该标签可用作样品中存在的微生物种类的定量和定性指标。

9900301, AzamA methodology is presented that, if successful, will simultaneously quantify and qualify the majority of microbes present in an environmental sample. Such a methodology is a prerequisite to studying the distribution and diversity ofmarine microbes on the ocean basin scale. The research method is based on the fact that a seven base pair DNA tag, if sufficiently divergent, can differentiate between thousands microbial species. To be useful this tag region must be invariably located within a microbe's genome and it must be associated with a conserved DNA region that can be used to isolate the tag A tag region within the RNase P locus, a ubiquitous nbozyme, that fulfills the criteria ofbemg highly divergent and located next to a conserved region is identified. Fortuitously, the RNase P locus is also always found as one copy per genome. Therefore, this tag can be used as a quantitative, as well as qualitative, indicator of the microbial species present in a sample.