SGER: A Rapid Method for Simultaneously Quantifying and Identifying Marine Microbes

SGER：同时定量和识别海洋微生物的快速方法

• 批准号: 9900301
• 负责人: Farooq Azam
• 金额: $ 5万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9900301&HistoricalAwards=false
• 关键词: SGER; Rapid; Method; Simultaneously; Quantifying

9900301, AzamA methodology is presented that, if successful, will simultaneously quantify and qualify the majority of microbes present in an environmental sample. Such a methodology is a prerequisite to studying the distribution and diversity ofmarine microbes on the ocean basin scale. The research method is based on the fact that a seven base pair DNA tag, if sufficiently divergent, can differentiate between thousands microbial species. To be useful this tag region must be invariably located within a microbe's genome and it must be associated with a conserved DNA region that can be used to isolate the tag A tag region within the RNase P locus, a ubiquitous nbozyme, that fulfills the criteria ofbemg highly divergent and located next to a conserved region is identified. Fortuitously, the RNase P locus is also always found as one copy per genome. Therefore, this tag can be used as a quantitative, as well as qualitative, indicator of the microbial species present in a sample.

9900301，提出了 AzamA 方法，如果成功，将同时量化和鉴定环境样本中存在的大多数微生物。这种方法是研究洋盆尺度海洋微生物的分布和多样性的先决条件。该研究方法基于这样一个事实：七碱基对 DNA 标签如果足够不同，可以区分数千种微生物物种。为了有用，这个标签区域必须始终位于微生物的基因组内，并且它必须与可用于分离 RNase P 基因座内的标签 A 标签区域相关的保守 DNA 区域，这是一种普遍存在的 nbozyme，满足高度分歧的标准，并且位于保守区域旁边。幸运的是，RNase P 基因座也总是每个基因组有一个拷贝。因此，该标签可用作样品中存在的微生物物种的定量和定性指示剂。