Artificial Extracellular Matrix Proteins

人工细胞外基质蛋白

• 批准号: 9901648
• 负责人: David Tirrell
• 金额: $ 32.96万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9901648&HistoricalAwards=false
• 关键词: Artificial; Extracellular; Matrix; Proteins

The objective of this project is to design and synthesize a new class of protein biomaterials for use in vascular graft applications. The proteins consist of repeats of elastin derived polypeptides with various cell binding peptides. They will be produced by expressing artificial genes in bacteria. The molecular structure of the materials will be manipulated via peptide sequence modifications and crosslinking methods in order to optimize mechanical and cell interaction properties. Finally, the attachment, spreading and function of vascular endothelial and smooth muscle cells on these materials will be evaluated under both static and perfused culture conditions, as a function of molecular properties.

本项目的目的是设计和合成一类新的蛋白质生物材料用于血管移植物的应用。 这些蛋白质由弹性蛋白衍生的多肽与各种细胞结合肽的重复序列组成。 它们将通过在细菌中表达人工基因来生产。 将通过肽序列修饰和交联方法操纵材料的分子结构，以优化机械和细胞相互作用特性。 最后，将在静态和灌注培养条件下评价血管内皮细胞和平滑肌细胞在这些材料上的附着、扩散和功能，作为分子特性的函数。