Characterization of a Limb Autotomy Factor, LAFpro, That Suspends Molting in Crustaceans

暂停甲壳类动物蜕皮的肢体自切因子 LAFpro 的表征

• 批准号: 9904528
• 负责人: Donald Mykles
• 金额: $ 27.36万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9904528&HistoricalAwards=false
• 关键词: Characterization; Limb; Autotomy; Factor; LAFpro

Crabs and lobsters grow by periodically shedding the hard exoskeleton through a process called molting. Molting and limb regeneration is regulated by hormones produced by endocrine organs located in the eyestalks and body. Previous studies, however, indicate that control of molting is more complex. If a regenerating walking leg (limb bud) is removed (autotomized) before the animal molts, molting is delayed 2-3 weeks, which allows time for the lost tissue to be regenerated. Dr. Mykles and associates have shown that limb buds produce a molt-inhibiting protein, termed limb autotomy factor - proecdysis (LAFpro). The overall goal of this project is to understand how this factor suspends molting processes. The proposal consists of three specific aims: (1) to isolate LAFpro from limb buds; (2) to determine the mechanism of LAFpro action on target organs; and (3) to determine the role of the nervous system in mediating the response to tissue regeneration.This research has broad application to understanding the control of growth in arthropods as a whole. Suspension of molting by tissue regeneration is not unique to crustaceans. Regeneration also delays molting in a variety of insects. This suggests a common mechanism. If so, he should be able develop entirely new strategies for controlling insect pests.

螃蟹和龙虾通过“蜕皮”的过程周期性地脱落坚硬的外骨骼来生长。 蜕皮和肢体再生受到位于眼柄和身体的内分泌器官产生的激素的调节。 然而，先前的研究表明，蜕皮的控制更为复杂。 如果在动物蜕皮之前将再生的步行腿（肢芽）切除（自动切除），则蜕皮会延迟 2-3 周，从而为失去的组织提供再生时间。 Mykles 博士及其同事证明，肢芽会产生一种蜕皮抑制蛋白，称为肢体自切因子 - 蜕皮 (LAFpro)。该项目的总体目标是了解该因素如何暂停蜕皮过程。 该提案包括三个具体目标：（1）从肢芽中分离LAFpro； (2)确定LAFpro作用靶器官的机制； （3）确定神经系统在介导组织再生反应中的作用。这项研究对于理解节肢动物整体生长的控制具有广泛的应用。 通过组织再生来暂停蜕皮并不是甲壳类动物所独有的。 再生也会延迟多种昆虫的蜕皮。 这表明了一个共同的机制。 如果是这样，他应该能够开发出全新的控制害虫的策略。