Upgrade of a Bruker AMX 500 MHz Nuclear Magnetic Resonance Spectrometer

Bruker AMX 500 MHz 核磁共振波谱仪的升级

• 批准号: 9908399
• 负责人: Lila Gierasch
• 金额: $ 5万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9908399&HistoricalAwards=false
• 关键词: Upgrade; Bruker; AMX; 500; MHz

9908399Abstract This project involves the upgrade of a 500 MHz NMR spectrometer to provide state-of-the-art capabilities in order to support the research of a group of users in the Department of Chemistry at University of Massachusetts, Amherst. The major research projects will be facilitated by the availability of the new instrumentation. This research will include determination of the structure of the peptide-binding domains of the molecular chaperones DnaK and BiP; determination of bound conformation of signal sequences upon interaction with their receptors; determination of the structure of an SRP-binding RNA fragment alone and in complex with a polypeptide; studies of the mechanism of folding of the predominantly beta-sheet protein, cellular retinoic acid biding protein; structural analysis of myoglobin mutants as models for guanylate cyclase; conformational studies of model helical peptides; determination of the active site geometry of metalloproteins; determination of the structure and dynamics of membrane proteins, including E. coli chemotactic receptors and colicin A; studies of domains of the E. Coli chemotactic receptor that are involved in adaptation; and determination of the structures of ribosomal proteins and the RNAs to which they bind. Additional projects will further utilize the remaining time on this instrument.

9908399Abstract 该项目涉及升级 500 MHz NMR 波谱仪，以提供最先进的功能，以支持马萨诸塞大学阿默斯特分校化学系的一组用户的研究。 新仪器的使用将促进重大研究项目的开展。 这项研究将包括确定分子伴侣 DnaK 和 BiP 的肽结合域的结构；确定信号序列与受体相互作用时的结合构象； 确定单独的SRP结合RNA片段和与多肽复合的SRP结合RNA片段的结构；研究主要β-折叠蛋白、细胞视黄酸结合蛋白的折叠机制；作为鸟苷酸环化酶模型的肌红蛋白突变体的结构分析；模型螺旋肽的构象研究；确定金属蛋白活性位点的几何形状；确定膜蛋白的结构和动力学，包括大肠杆菌趋化受体和大肠杆菌素 A；研究参与适应的大肠杆菌趋化受体结构域；核糖体蛋白及其结合 RNA 的结构测定。 其他项目将进一步利用该工具的剩余时间。