Photorespiration-centred metabolic modelling: reconstruction and structural analysis of the network of primary metabolism in cyanobacteria, comparison to eukaryotic energy and central carbon metabolism, metabolic engineering approach for optimisation of C

以光呼吸为中心的代谢模型：蓝藻初级代谢网络的重建和结构分析，与真核能量和中心碳代谢的比较，优化C的代谢工程方法

• 批准号: 134778053
• 负责人: Professor Dr. Olaf Wolkenhauer
• 金额: --
• 依托单位: Lehrstuhl für Systembiologie und Bioinformatik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/134778053?language=en
• 关键词: Photorespiration; centred; metabolic; modelling; reconstruction

The purpose of the cooperation between modelers and experimentalists in this project is to apply computational approaches to improve our understanding of photosynthetic carbon metabolism, particularly its efficiency and regulation in connection with the photorespiratory pathway. We can build both on our previous study of the Calvin-Benson cycle and adjacent pathways as well as on the results coming from our collaboration with Hagemann group. This work focuses on the redirection of carbon flux after changing from high to low CO2 in the case of Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803. The next funding period would concentrate on identifying the rate-limiting steps in the transport-chain and critical metabolic regulators in cyanobacteria and C3 plants (and optionally in C4 plants) which will require multi-level modeling approach, integrating metabolic and transcriptomic data, photorespiratory knockout mutants, labelling experiments and overexpression. The modeling efforts will start with non-compartmental representation which will identify possible rate limitations in the transporter-chain among compartments and separated pools of metabolites. Finally, comparison between cyanobacterial photorespiratory mutant without the carboxysome, C3 and C4 carbon fixations can provide new insight into efficiency of the carbon metabolism.

在这个项目中，建模者和实验者之间合作的目的是应用计算方法来提高我们对光合碳代谢的理解，特别是其与光呼吸途径相关的效率和调节。我们可以建立在我们以前对卡尔文-本森循环和邻近途径的研究以及我们与哈格曼小组合作的结果的基础上。本工作的重点是改变后，从高到低CO2的细长聚球藻PCC 7942和集胞藻属PCC 6803的情况下，碳通量的重定向。下一个资助期将集中于确定蓝细菌和C3植物（以及可选的C4植物）中运输链和关键代谢调节剂的限速步骤，这将需要多级建模方法，整合代谢和转录组学数据，光呼吸敲除突变体，标记实验和过表达。建模工作将从非房室模型开始，这将确定房室和分离的代谢物池之间转运链中可能的速率限制。最后，比较没有羧基体、C3和C4碳固定的蓝藻光呼吸突变体，可以为碳代谢效率提供新的见解。