Identification and investigation of osmostress-induced genes in Dictyostelium

几乎所有的细胞，甚至是多细胞生物体中的单个细胞，都会受到渗透环境的影响，有时变化非常迅速。为了生存，细胞必须感知这些变化，并引发适当的反应，使它们能够适应。对渗透胁迫反应的全面看法需要对细胞的各个方面，如信号传感和转导，运输过程和代谢的控制以及转录和翻译的差异调节的详细理解。盘基网柄藻是大规模研究转录和翻译适应变化环境的一个强大的模型系统。它是特别适合的，因为与高等真核生物的高度进化保守，最先进的军械库的分子遗传技术，以调查相关基因和完整的基因组序列和DNA微阵列的可用性。我们在这个项目中的目标是进一步分析由高渗激活的STATc信号通路，也分析响应于低渗条件下的转录变化，并阐明两种反应之间的共性和差异。基于我们在之前的研究结果，我们将产生和分析敲除和过表达STATc途径的可疑关键信号基因的菌株。这种方法应使我们能够进一步阐明的作用，他STATc通路在高渗反应，在这种较低的真核生物，并比较其组件与高等真核生物。

Virtually all cells, even individual cells in multi-cellular organisms, are subject to changes in the osmotic environment that are sometimes extremely rapid. In order to survive cells have to sense these changes and to elicit an appropriate response that allows them to adapt. A comprehensive view on the osmotic stress response requires a detailed understanding of various cellular aspects such as signal sensing and transduction, control of transport processes and metabolism and differential regulation of transcription and translation. Dictyostelium discoideum is a powerful model system for large-scale studies of the transcriptional and translational „ adaptations to a changing environment. It is particularly suited because of the high degree of evolutionary conservation with higher eukaryotes, the state of the art armoury of molecular genetic techniques to investigate relevant genes and the availability of the complete genome sequence and of DNA microarrays. Our goals in this project are to further analyse the STATc signalling pathway activated by hyperosmolarity, to also analyse transcriptional changes in response to hypoosmotic conditions and to elucidate the commonalities and differences between the two responses. Based on our results in the previous grant we will generate and analyse knock-out and over-expressing strains of suspected key signalling genes of the STATc pathway. This approach should allow us to further elucidate the role oft he STATc pathway in response to hypertonicity in this lower eukaryote and to compare its components with those of higher eukaryotes.