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Stoichiometric Shifting of the Plant Mitochondrial Genome

植物线粒体基因组的化学计量变化

基本信息

批准号：
9974799
负责人：
Sally Mackenzie
金额：
$ 5万
依托单位：
University of Nebraska-Lincoln
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1999
资助国家：
美国
起止时间：
1999-08-01 至 2000-10-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9974799&HistoricalAwards=false
关键词：
Stoichiometric Shifting Plant Mitochondrial Genome

项目摘要

The plant mitochondrial genome has evolved several distinguishing features that influence its structure and expression. One of these is the maintenance of subgenomic DNA molecules at markedly different copy numbers. The phenomenon of genomic shifts in copy number and the retention of substoichiometric DNA molecules within the plant mitochondrial genome will be investigated using the cytoplasmic male sterility system in common bean. This award will support two graduate students to complete their Ph.D. thesis research in two areas of this topic. The first will investigate the physiological implications of genomic shifts in the mitochondrial genome by examining the effects of shifting on cross-compatibility, the influence of temperature on the shifting process, and the plant tissues in which the mitochondrial genome is protected from shifting. The second student will investigate the expression of the mitochondrial sterility sequence, pvs-orf239, and its influence on the shifting process. This will include investigation of the export of the ORF239 product from the mitochondrion to the periphery of the cell; these particular studies will be carried out in yeast transformed with the pvs-orf239 sequence.These investigations, through taking advantage of features special to the common bean system, address biological phenomena relevant to all higher plants, and should provide strategies for directed manipulation of the plant mitochondrial genotype.

植物线粒体基因组进化出了几个影响其结构和表达的显著特征。其中之一是保持亚基因组DNA分子的拷贝数明显不同。利用菜豆细胞质雄性不育系统研究植物线粒体基因组中拷贝数的改变和亚化学计量比DNA分子的保留现象。该奖项将支持两名研究生完成这一主题的两个领域的博士论文研究。第一部分将通过研究移位对交叉亲和性的影响，温度对移位过程的影响，以及保护线粒体基因组免受移位的植物组织来研究线粒体基因组移位的生理意义。第二个学生将研究线粒体不育序列pvs-orf239的表达及其对转变过程的影响。这将包括调查ORF239产物从线粒体到细胞外围的出口；这些特殊的研究将在转化了pVS-orf239序列的酵母中进行。这些研究通过利用菜豆系统特有的特征，解决与所有高等植物相关的生物学现象，并应提供定向操纵植物线粒体基因的策略。