EAGER: Documenting Epigenetic Influence on Plant Quantitative Phenotypic Variation
EAGER:记录表观遗传对植物数量表型变异的影响
基本信息
- 批准号:1853519
- 负责人:
- 金额:$ 30万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-11-15 至 2021-10-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A major challenge to the stability of US and worldwide agricultural is the increasing variability of weather conditions coupled with diminishing water availability. These challenges require the development of greater plant resiliency that, in an agricultural context, can be scaled to meet US needs and can be implemented in multiple crops. Conventional breeding enhances plant yield by selecting for enhanced genetics that can withstand various environmental stresses (e.g., drought). Similar gains might also be influenced by epigenetics (modifications to DNA). However, using epigenetics to enhance plant resilience requires formal demonstration and the development of appropriately sensitive computational technologies to monitor epigenetic changes across the plant genome. This project tests using epigenetics to enhance plant by using a new genetic mutation that leads to epigenetic changes in plants. Some plants with this mutation show enhanced growth and resilience that is maintained after the mutation is removed and this project will generate new computational tools to analyze these long-lasting (multi-generational) effects. Due to the potential for transferring this technology to crop species, this technique will have broad impacts on plant research and crop improvement. Accelerated plant adaptation to environmental change requires adjustment of gene expression in a manner that is ultimately heritable to offspring, yet little is known of the mechanism of transgenerational epigenetic behavior. This project addresses this gap by exploiting the MSH1 system in Arabidopsis for inducible transgenerational memory and phenotypic plasticity. A novel Methyl-IT program for genome-wide methylation analysis will be used to contrast DNA methylation patterns in full sib progeny with and without induced epigenetic memory effects to test for a direct relationship between cytosine methylation behavior and phenotype emergence. Graft progeny out-comes from wild type scion/msh1 rootstock will be tested for epigenetic effects by introducing mutations for sRNA production to the rootstock genotype and assaying progeny for alignment in sRNA, methylome and phenotype changes. Outcomes from this study will permit assessment of epigenome-phenotype relationship by direct testing. Breaking through this barrier has the potential to dramatically alter current thinking in the field, and designing experimental methods for the detection of subtle, gene-associated cytosine methylation variation would impact epigenomic re-search in both plant and animal systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
对美国和世界农业稳定性的一个主要挑战是天气条件的变化越来越大,加上水资源的可用性越来越少。这些挑战需要开发更大的植物弹性,在农业背景下,可以扩展以满足美国的需求,并可以在多种作物中实施。 常规育种通过选择能够承受各种环境胁迫(例如,干旱)。 类似的收益也可能受到表观遗传学(DNA修饰)的影响。 然而,利用表观遗传学来增强植物的适应能力需要正式的论证和开发适当敏感的计算技术来监测整个植物基因组的表观遗传变化。 该项目测试使用表观遗传学,通过使用一种新的基因突变,导致植物表观遗传学的变化,以提高植物。 一些具有这种突变的植物表现出增强的生长和恢复力,这些生长和恢复力在突变被去除后仍能保持,该项目将产生新的计算工具来分析这些长期(多代)影响。 由于这项技术有可能转移到作物品种上,这项技术将对植物研究和作物改良产生广泛的影响。加速植物对环境变化的适应需要以最终可遗传给后代的方式调整基因表达,但对跨代表观遗传行为的机制知之甚少。 该项目通过利用拟南芥中的MSH 1系统来解决这一差距,以诱导跨代记忆和表型可塑性。将使用一种用于全基因组甲基化分析的新型Methyl-IT程序来对比具有和不具有诱导的表观遗传记忆效应的全同胞后代中的DNA甲基化模式,以测试胞嘧啶甲基化行为与表型出现之间的直接关系。将通过将sRNA产生的突变引入砧木基因型并测定子代的sRNA、甲基化组和表型变化的比对来测试来自野生型接穗/mshl砧木的嫁接子代产物的表观遗传效应。本研究的结果将允许通过直接测试评估表观基因组-表型关系。突破这一障碍有可能极大地改变该领域目前的思维,设计检测微妙的基因相关胞嘧啶甲基化变异的实验方法将影响植物和动物系统中的表观基因组研究。该奖项反映了NSF的法定使命,并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming
- DOI:10.1038/s41467-020-16036-8
- 发表时间:2020-05-05
- 期刊:
- 影响因子:16.6
- 作者:Yang, Xiaodong;Sanchez, Robersy;Mackenzie, Sally A.
- 通讯作者:Mackenzie, Sally A.
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Sally Mackenzie其他文献
Sally Mackenzie的其他文献
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{{ truncateString('Sally Mackenzie', 18)}}的其他基金
Intersection of the Plant Epigenome and Bioenergetics in Phenotypy
植物表观基因组与表型生物能学的交叉点
- 批准号:
1126935 - 财政年份:2011
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
2009 International Conference on Plant Mitochondrial Biology (ICPMB), Lake Tahoe, California
2009 年国际植物线粒体生物学会议 (ICPMB),加利福尼亚州太浩湖
- 批准号:
0907861 - 财政年份:2009
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
TRMS: An Integrative Study of Plant Mitochondrial Biology
TRMS:植物线粒体生物学的综合研究
- 批准号:
0820668 - 财政年份:2008
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Nuclear Mechanisms that Influence Mitochondrial Genome Stability
影响线粒体基因组稳定性的核机制
- 批准号:
0744104 - 财政年份:2008
- 资助金额:
$ 30万 - 项目类别:
Continuing Grant
The Machinery of Mitochondrial Recombination in Higher Plants
高等植物线粒体重组机制
- 批准号:
0417172 - 财政年份:2004
- 资助金额:
$ 30万 - 项目类别:
Continuing Grant
Mitochondrial Sorting and Inheritance in Arabidopsis
拟南芥线粒体分选和遗传
- 批准号:
0323377 - 财政年份:2003
- 资助金额:
$ 30万 - 项目类别:
Continuing Grant
Investigating Mitochondrial Genome Dynamics in Plants
研究植物线粒体基因组动力学
- 批准号:
0090813 - 财政年份:2001
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Stoichiometric Shifting of the Plant Mitochondrial Genome
植物线粒体基因组的化学计量变化
- 批准号:
9974799 - 财政年份:1999
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Nuclear Regulation of Mitochondrial Genome Integrity
线粒体基因组完整性的核调控
- 批准号:
9996325 - 财政年份:1999
- 资助金额:
$ 30万 - 项目类别:
Continuing Grant
Mitochondrial Factors Associated with Pollen Development
与花粉发育相关的线粒体因素
- 批准号:
9896395 - 财政年份:1999
- 资助金额:
$ 30万 - 项目类别:
Continuing Grant
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