Acquisition of High-resolution Multi-mode Digital Microscope System for Biological Research

乔治兰福德达特茅斯学院获得用于生物学研究的高分辨率多模式数字显微镜系统该奖项将用于购买具有多光学模式的研究质量显微镜。该显微镜将用于获得荧光探针染色的活细胞的定量信息。一种具有多模显微镜系统功能的仪器目前还没有提供给达特茅斯学院的教职员工和学生。该系统特别适合于跟踪活细胞中与绿色荧光蛋白（GFP）融合的蛋白质。使用显微镜的具体项目包括细胞骨架动力学的研究。特别是，通过x -罗丹明与微管蛋白或肌动蛋白偶联标记的间期和纺锤体微管和肌动蛋白丝，以及活细胞内质网膜（gfp标记的内质网蛋白）和细胞皮层（通过差分干涉对比检测；DIC）的动力学将被研究。将对细胞的GFP和DIC图像进行叠加，以确定细胞骨架和内质网膜之间的相互作用。这台显微镜将提供给教师在生物科学系和分子和细胞生物学研究生课程。除了研究之外，这台仪器还将用于培训本科生、研究生和博士后，包括达特茅斯学院“女性参与科学项目”和欧内斯特·埃弗雷特·杰斯特科学项目的参与者。

Award Abstract9977613George LangfordDartmouth College Acquisition of High-resolution Multi-mode Digital Microscope System for Biological Research This award will be used to purchase a research quality microscope with multiple optical modes. This microscope will be used to obtain quantitative information from living cells stained with fluorescent probes. An instrument with the capabilities of the multi-mode microscope system is not currently available to the faculty and students at Dartmouth College. This system is particularly suited for tracking proteins fused to the green fluorescent protein (GFP) in living cells. The specific projects for which the microscope will be used include the study of the dynamics of the cytoskeleton. In particular, interphase and spindle microtubules and actin filaments labeled by conjugating X-rhodamine to tubulin or actin as well as the dynamics of membranes of the endoplasmic reticulum (GFP-tagged ER proteins) and the cell cortex (detected by differential interference contrast; DIC) in living cells will be studied. Overlay of GFP and DIC images of cells will be performed to determine the interactions between the cytoskeleton and membranes of the ER.This microscope will be available to the faculty in the Department of Biological Sciences and the Molecular and Cell Biology Graduate Program. In addition to research, the instrument will be used to train undergraduate and graduate students and postdoctoral fellows, including participants in Dartmouth's Women in Science Program and the Ernest Everett Just program for African-American students in the Sciences.