Development of a Very High Field Solid-state NMR Spectrometer

超高场固态核磁共振波谱仪的研制

• 批准号: 9977682
• 负责人: Stanley Opella
• 金额: --
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-10-01 至 2001-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9977682&HistoricalAwards=false
• 关键词: Development; Very; Field; Solid; state

9977682OpellaThis project is to develop a very high field NMR spectrometer that will be dedicated to the determination of structure of proteins in biological supra-molecular structures, including membranes and virus particles. The spectrometer will operate at the highest available resonance frequencies and will perform the full range of high-resolution solid-state NMR experiments needed for the resolution, assignment and measurement of spectroscopic parameters of individual resonances from uniformly labeled protein samples. The structures of membrane proteins, in particular, are vastly under-represented among known structures in the Protein Data Bank because of the technical difficulties they present for conventional x-ray crystallographic and solution NMR methods. These proteins do not crystallize and reorient too slowly in solution. The ability of NMR to determine protein structure, characterize dynamics and describe chemical properties and interactions depends on the capabilities of the electronic components and, especially, the strength of the applied magnetic field. The advantages of using the strongest available magnet are disproportionately large because of the associated synergistic improvements in sensitivity, resolution and control.The group plans to purchase the highest field magnet available, and to develop the associated instrumentation, especially the probes, for a research spectrometer optimized for protein structure determination by solid state NMR spectroscopy. Preliminary results demonstrate the feasibility of constructing and tuning high power double-and triple-resonance probes for frequencies in the range of 700 to 1100 MHz. There will be substantial impact on future generations of spectroscopists because much of the development and experimental work will be performed by graduate students and postdoctoral research associates at the Resource for Solid-State NMR of Proteins at the University of Pennsylvania.

9977682Opella这个项目是开发一个非常高的领域NMR光谱仪，将致力于确定生物超分子结构中的蛋白质结构，包括膜和病毒颗粒。 该光谱仪将在最高可用共振频率下运行，并将进行全方位的高分辨率固态NMR实验，这些实验是从统一标记的蛋白质样品中分辨、分配和测量各个共振的光谱参数所需的。 特别是膜蛋白的结构，在蛋白质数据库中的已知结构中代表性大大不足，因为它们为传统的X射线晶体学和溶液NMR方法带来了技术困难。 这些蛋白质在溶液中不会结晶和重新定向得太慢。 NMR确定蛋白质结构、表征动力学和描述化学性质和相互作用的能力取决于电子元件的能力，特别是所施加磁场的强度。 由于灵敏度、分辨率和控制方面的协同改进，使用最强磁体的优势不成比例地大。该集团计划购买最强磁场磁体，并开发相关仪器，特别是探针，用于研究光谱仪，优化固态NMR光谱法测定蛋白质结构。 初步结果表明，在700至1100 MHz的范围内的频率构建和调谐高功率双和三谐振探头的可行性。 这将对未来几代光谱学家产生重大影响，因为大部分开发和实验工作将由宾夕法尼亚大学蛋白质固态NMR资源的研究生和博士后研究人员进行。