Spatial Scales of Genetic and Phenotypic Diversity Among Streptomycetes in Native Soils

原生土壤中链霉菌遗传和表型多样性的空间尺度

• 批准号: 9977907
• 负责人: Linda Kinkel
• 金额: $ 99万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9977907&HistoricalAwards=false
• 关键词: Spatial; Scales; Genetic; Phenotypic; Diversity

9977907Kinkel, L.Samac, D.This Microbial Observatory will provide fundamental information on the genetic and phenotypic diversity among Streptomycetes bacteria occurring in native soils at Cedar Creek Natural History Area, a Long Term Ecological Research site at the forest-prairie interface in central Minnesota. In particular, this work will provide significant insight into the dynamics of interisolate antibiotic inhibition, resistance, and induction within these communities, as well as a spatial, temporal, and genetic context for analyzing these phenotypic characters. The influence of soil nitrogen treatment on the community will also be evaluated here. The specific objectives of the research are to: 1. Evaluate the influence of nitrogen inputs on the Streptomycete community in soil. This will be done by contrasting the population densities of Streptomycetes in soils maintained at two different nitrogen input levels; contrasting the diversity of Streptomycete communities in soils maintained at the two nitrogen levels, specifically focusing on genetic diversity and diversity in antibiotic inhibition and resistance. 2. Quantify the genetic and phenotypic diversity in Streptomycetes across a range of spatial scales; quantifying genetic diversity among Streptomycetes in individual soil cores, in separate cores from the same plot, and from different plots; quantifying diversity in inhibition profiles among Streptomycetes in individual soil cores, in separate cores from the same plot, and from different plots; and quantifying diversity in resistance profiles among Streptomycetes in individual soil cores, in separate cores from the same plot, or from different plots. 3. Determine the influence of spatial origin, population density of the local community, and genetic relatedness on the potential for inhibition, resistance to inhibition, and antibiotic induction among Streptomycetes. This will by done by determining whether isolates from the same location (soil core), same plot, or same nitrogen treatment are more or less likely to inhibit, to resist inhibition by, or to induce antibiotic production than isolates from different soil cores, different plots, or different nitrogen treatments; determining whether isolates that are genetically closely related are more or less likely to inhibit one another, to resist inhibition by one another, or to induce antibiotic production in one another than isolates that are more distantly related; and determining whether isolates from high density locations in soil are more or less likely to inhibit other isolates, resist inhibition by others, or induce antibiotic production in others than isolates from low density locations. This information will provide fundamental insight into the potential significance of antibiotic interactions for evolution and microbial population dynamics in soils.

9977907Kinkel，L.Samac，d。该微生物观测站将提供有关在明尼苏达州中部森林植物群的长期生态研究地点的雪松溪自然历史区域的链霉菌细菌中链霉菌细菌的遗传和表型多样性的基本信息。特别是，这项工作将为这些社区内的跨性别抗生素抑制，抗性和诱导的动力学提供重大见解，以及用于分析这些表型特征的空间，时间和遗传环境。这里还将评估土壤氮处理对社区的影响。该研究的具体目标是：1。评估氮输入对土壤中链霉菌群社区的影响。 这将通过将维持在两个不同氮输入水平的土壤中的链霉菌的种群密度进行对比。 将维持在两个氮水平的土壤中链霉菌群落的多样性进行了对比，特别关注抗生素抑制和抗性的遗传多样性和多样性。 2。量化一系列空间尺度的链霉菌中的遗传和表型多样性；量化各个土壤核心链霉菌之间的遗传多样性，与同一地块的单独核心以及不同的地块中的遗传多样性；量化单个土壤核心链霉菌之间的抑制谱的多样性，与同一地块的单独核心和不同地块的单独岩心中的多样性；并量化各个土壤核心链霉菌，与同一地块或不同图的单独岩心的链霉菌之间的抗性谱。 3。确定空间起源，当地社区的种群密度以及遗传相关性对链霉菌中抑制作用，抗抑制作用和抗生素诱导的可能性的影响。 通过确定从同一位置（土壤核心），相同的地块或相同氮处理的分离株来抑制或多或少地抑制与来自不同土壤核心，不同土地不同的土壤或不同氮处理的抗生素产生的可能性或多或少；确定遗传上密切相关的分离株是否或多或少地抑制彼此的抑制作用，或者彼此抑制，或者互相诱导抗生素的产生，而不是遥远相关的分离株。并确定来自土壤中高密度位置的分离株是否或多或少地抑制其他分离株，抵抗他人的抑制作用或诱导其他分离物的产生，而不是从低密度位置的分离物中抑制抗生素。 该信息将提供对抗生素相互作用对土壤进化和微生物种群动态的潜在意义的基本见解。