Rice Genome Sequencing Project

水稻基因组测序计划

• 批准号: 9982594
• 负责人: Richard Hilderman
• 金额: $ 340万
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-10-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982594&HistoricalAwards=false
• 关键词: Rice; Genome; Sequencing; Project

The cereals are the most important food crops in the world. Determining the entire genomic sequence of a model cereal will reveal the basic gene set from monocot plants. This award will support an experienced and productive team to participate in an international effort to determine the genomic blueprint of rice. The project will involve sequencing and annotation of approximately 21 Mb of contiguous rice genomic sequence over 3 years. Initial emphasis will be placed on chromosome 10. The overall project will be managed by Clemson University (CUGI) in collaboration with Cold Spring Harbor Laboratory (CSHL) and the Washington University (WUGSC). 160 130kb Nipponbare BAC clones anchored to a known chromosome site will be validated for insert integrity and chromosome location by CUGI. Shotgun library development and production sequencing of these clones will be performed at CSHL (64%) and CUGI (33%). Finishing and automated annotation will occur at both sites. WUGSC will lead a group in finishing technology development aimed at establishing robust chemistries that can be used by the consortium to finish difficult regions of the rice genome. All sequence data will be immediately released to community databases (Genbank HTGS section) according to the "Bermuda" policies. In addition, results will be published in scientific journals and annotated BAC clones will be displayed in an electronic form accessible to the scientific community.

谷物是世界上最重要的粮食作物。 确定模式谷物的整个基因组序列将揭示单子叶植物的基本基因组。 该奖项将支持一个经验丰富和富有成效的团队参与国际努力，以确定水稻的基因组蓝图。 该项目将在3年内对大约21 Mb的连续水稻基因组序列进行测序和注释。 最初的重点将放在10号染色体上。 整个项目将由克莱姆森大学（CUGI）与冷泉港实验室（CSHL）和华盛顿大学（WUGSC）合作管理。将通过CUGI验证锚定到已知染色体位点的160个130kb Nipponbare BAC克隆的插入完整性和染色体定位。这些克隆的鸟枪文库开发和生产测序将在CSHL（64%）和CUGI（33%）进行。 两个研究中心都将进行精加工和自动注释。 WUGSC将领导一个整理技术开发小组，旨在建立强大的化学物质，可供该财团用于完成水稻基因组的困难区域。 根据“百慕大”政策，所有序列数据将立即发布到社区数据库（Genbank HTGS部分）。 此外，研究结果将发表在科学期刊上，并将以电子形式向科学界展示附有注释的BAC克隆。