Rice Genome Sequencing Project

点击翻译按钮获取中文摘要

The cereals are the most important food crops in the world. Determining the entire genomic sequence of a model cereal will reveal the basic gene set from monocot plants. This award will support an experienced and productive team to participate in an international effort to determine the genomic blueprint of rice. The project will involve sequencing and annotation of approximately 21 Mb of contiguous rice genomic sequence over 3 years. Initial emphasis will be placed on chromosome 10. The overall project will be managed by Clemson University (CUGI) in collaboration with Cold Spring Harbor Laboratory (CSHL) and the Washington University (WUGSC). 160 130kb Nipponbare BAC clones anchored to a known chromosome site will be validated for insert integrity and chromosome location by CUGI. Shotgun library development and production sequencing of these clones will be performed at CSHL (64%) and CUGI (33%). Finishing and automated annotation will occur at both sites. WUGSC will lead a group in finishing technology development aimed at establishing robust chemistries that can be used by the consortium to finish difficult regions of the rice genome. All sequence data will be immediately released to community databases (Genbank HTGS section) according to the "Bermuda" policies. In addition, results will be published in scientific journals and annotated BAC clones will be displayed in an electronic form accessible to the scientific community.