Design of a bioinformatic database for functional evolutionary footprints in multigene families

多基因家族功能进化足迹生物信息数据库的设计

• 批准号: 9983133
• 负责人: Sudhir Kumar
• 金额: $ 67.74万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9983133&HistoricalAwards=false
• 关键词: Design; bioinformatic; database; functional; evolutionary

Understanding the molecular basis for origin of new function and diversificaton of existing gene function is of fundamental importance in molecular biology. Gene duplications are possibly the predominant mechanism for generating raw materials for functional evolution, as the duplicate copies may acquire a new function by accumulating mutational changes (substitutions) in their coding sequences. Biochemical properties and the location of such changes in the protein sequence are the evolutionary "footprints" of functional diversification. These mutations are under strong purifying selection pressures to maintain the novel/modified function. The goal of this project is to design a biological database and an informatic system with the following objectives:1. Design and construction of a database of potentially functionally important mutations in animal multigene families to facilitate identification and exploration of the evolutionary footprints in the following contexts: (i) protein sequence context [e.g., alpha-helix], (ii) duplicate gene function context [e.g., DNA-binding activity], and (iii) phylogenetic context of gene duplication events [e.g., vertebrate-invertebrate divergence, radiation of mammals].2. Development of a general purpose Web-based client/server resource for researchers world-wide to dynamically and interactively query, retrieve, explore, and analyze evolutionary footprints.The proposed Evolutionary Footprint (EF) Client/Server System will be useful for researchers interested in exploring evolutionary footprints and will address the day-to-day needs of researchers in many different areas of molecular biology including computational genomics, evolutionary biology, immunology, systematics, and developmental biology.

了解新功能起源和现有基因功能多样化的分子基础对于分子生物学至关重要。基因复制可能是生成功能进化原材料的主要机制，因为复制副本可能通过在其编码序列中积累突变变化（取代）来获得新功能。生化特性和蛋白质序列中此类变化的位置是功能多样化的进化“足迹”。这些突变处于强大的纯化选择压力下以维持新颖/修饰的功能。本项目的目标是设计一个生物数据库和信息系统，其目标如下： 1．设计和构建动物多基因家族中潜在功能重要突变的数据库，以促进在以下背景下识别和探索进化足迹：（i）蛋白质序列背景[例如α螺旋]，（ii）重复基因功能背景[例如DNA结合活性]，以及（iii）基因重复事件的系统发育背景[例如， 脊椎动物与无脊椎动物的分歧，哺乳动物的辐射].2。开发基于网络的通用客户端/服务器资源，供世界各地的研究人员动态和交互地查询、检索、探索和分析进化足迹。所提出的进化足迹（EF）客户端/服务器系统将对有兴趣探索进化足迹的研究人员有用，并将满足分子生物学许多不同领域的研究人员的日常需求，包括计算基因组学、进化生物学、免疫学、 系统学和发育生物学。