CAREER: Microdialysis Sampling Techniques to Assess and Improve Quantitative Sensocompatibility

这项由伦斯勒理工学院的Julie Stenken博士主持的职业研究计划得到了化学部分析和表面计划的支持。这项研究的重点是开发用于体外巨噬细胞培养的微透析探针，目的是实时监测化学浓度。需要监测的化学物质包括羟基自由基、一氧化氮和前列腺素。她将评估各种传感器膜材料的生物兼容性。她还将扩展她之前在环糊精络合方法上的工作，这种方法通过微透析采样来浓缩细胞培养中的微量化学物质。她研究的教育部分包括将这项研究纳入她的研究生课程。斯坦肯博士还计划为当地高中开发一个外展教育项目，专门针对代表性不足的女性和少数族裔学生。这项研究对生物医学工程界的影响预计会很大。Stenken博士将解决与微透析采样的常规使用相关的许多复杂问题。研究膜材料及其引起的炎症反应将使人们从根本上更清楚地了解材料与血液和组织的相容性。

This CAREER research program conducted by Dr. Julie Stenken of Rensselaer Polytechnic Institute is supported by the Analytical and Surface Program in the Chemistry Division. The focus of this research is to develop microdialysis probes for in vitro macrophage cell cultures with the goal of monitoring real-time chemical concentrations. The chemicals to be monitored include hydroxyl radical, nitric oxide and prostaglandins. She will evaluate the biocompatibility of a variety of sensor membrane materials. She also will expand her previous work on a cyclodextrin complexation method for concentrating trace chemicals in the cell culture with sampling by microdialysis. The educational component of her research includes the incorporation of this research into her graduate program. Dr. Stenken also plans to develop an outreach educational program for local high schools, directed specifically to underrepresented women and minority students.The impact of this research on the biomedical engineering community is expected to be high. There are a number of complex issues related to the routine use of microdialysis sampling which Dr. Stenken will address. Studying membrane materials and the inflammatory response which they cause will lead to a clearer fundamental understanding of the compatibility of materials with blood and tissues.