Development and integration of a cortisol sensor with real-time read-out to an ambulatory microdialysis sampling system

荷尔蒙皮质醇是在身体和心理压力下分泌的，影响身体的大多数组织。在健康情况下，它是按昼夜节律分泌的，但当采样足够频繁时，可以看到持续约一小时的极端脉搏节律。大幅度脉冲产生高，而小脉冲或没有脉冲产生低。身体读取这些脉冲--不仅是皮质醇的数量，而且是影响哪些基因被激活的模式。利用BBSRC的资金，我们开发了一种无血皮质醇采样设备。它需要频繁的样本，让我们能够看到皮质醇的脉搏。我们可以把它附加到一个人身上，他们就可以开始他们的生活了。目前，我们在实验室分析样本。我们想要开发一种用于皮质醇的传感器，以适应设备，这样结果就可以立即得到。我们已经确定了一种检测皮质醇的分子，但需要将其转化为有效的传感器，并将其与我们的收集设备集成在一起。

The hormone cortisol is secreted in response to physical and psychological stress and affects most tissues in the body. In health, it is secreted in a circadian rhythm, but when samples are taken frequently enough, an ultradian rhythm of pulses lasting about an hour can be seen. Large amplitude pulses produce the highs and small or no pulses the lows. The body reads these pulses - it is not just the amount of cortisol, but the pattern that affects which genes are activated. Using BBSRC funding, we developed a blood-free device for sampling cortisol. It takes frequent samples that allow us to see the pulses of cortisol. We can attach it to an individual and they go about their life. At the moment, we analyse the samples in a laboratory. We want to develop a sensor for cortisol to fit in the device, so that the results are immediately available. We have identified a molecule that detects cortisol, but need to turn this into a working sensor and integrate this with our collection device.