Development and integration of a cortisol sensor with real-time read-out to an ambulatory microdialysis sampling system

开发并集成具有实时读数功能的皮质醇传感器到动态微透析采样系统

• 批准号: BB/T004177/1
• 负责人: Stafford Lightman
• 金额: $ 81.63万
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT004177%2F1
• 关键词: Development; integration; cortisol; sensor; real

The hormone cortisol is secreted in response to physical and psychological stress and affects most tissues in the body. In health, it is secreted in a circadian rhythm, but when samples are taken frequently enough, an ultradian rhythm of pulses lasting about an hour can be seen. Large amplitude pulses produce the highs and small or no pulses the lows. The body reads these pulses - it is not just the amount of cortisol, but the pattern that affects which genes are activated. Using BBSRC funding, we developed a blood-free device for sampling cortisol. It takes frequent samples that allow us to see the pulses of cortisol. We can attach it to an individual and they go about their life. At the moment, we analyse the samples in a laboratory. We want to develop a sensor for cortisol to fit in the device, so that the results are immediately available. We have identified a molecule that detects cortisol, but need to turn this into a working sensor and integrate this with our collection device.

荷尔蒙皮质醇是对身体和心理压力的反应，影响身体的大多数组织。在健康情况下，它是按昼夜节律分泌的，但如果采样足够频繁，就可以看到持续约一小时的超昼夜脉冲节奏。大振幅脉冲产生高点，小脉冲或无脉冲产生低点。身体会读取这些脉冲——不仅仅是皮质醇的数量，而是影响哪些基因被激活的模式。在BBSRC的资助下，我们开发了一种检测皮质醇的无血设备。它需要频繁取样，让我们看到皮质醇的脉冲。我们可以把它附在一个人身上，然后他们开始自己的生活。目前，我们在实验室里分析样本。我们想要开发一种适合设备的皮质醇传感器，这样就可以立即获得结果。我们已经确定了一种可以检测皮质醇的分子，但需要将其转化为工作传感器，并将其与我们的收集设备集成在一起。