MRI: Acquisition of a Fluorescence Activated Cell Sorter for Biomedical & Bioscience Research and Training at University of Arkansas

MRI：获取用于生物医学的荧光激活细胞分选仪

• 批准号: 1337265
• 负责人: Julie Stenken
• 金额: $ 43.29万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337265&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluorescence; Activated; Cell

1337265JinThis proposal seeks to acquire a new fluorescence activated cell sorter (FACS), FACSAria III,to upgrade a multiuser-shared FACS that has been serving the entire campus of the University of Arkansas (UA) for more than 18 years. The requested new generation FACSAria III is capable of sorting four different types of cells and analyzing nine fluorescence markers simultaneously. Its rates of analysis and sorting are 75,000 events/second and 25,000 cells/second, respectively, with 98% of purity. This rapid sorting capability will significantly improve cell survival rate and reduce the chances of cell contamination. Access to this state-of-the-art flow cytometer and cell sorter will have an immediate and tremendous impact on UA researchers and students. Since flow cytometric analysis of cell types and further sorting them based on their properties are absolute pre-requisites for performing high-quality life science and biomedical research, FACSAria will propel UA?s recent health research initiative, boost faculty research capabilities in the newly-formed biomedical engineering department, and help more individuals on campus obtain preliminary results to seek NSF or NIH funding.

1337265 Jin该提案旨在获得一种新的荧光激活细胞分选仪（FACS），FACSAria III，以升级多用户共享的FACS，该FACS已服务于阿肯色州大学（UA）的整个校园超过18年。新一代FACSAria III能够分选四种不同类型的细胞，同时分析九种荧光标记物。它的分析和分选速度分别为75，000个事件/秒和25，000个细胞/秒，纯度为98%。这种快速分选能力将显著提高细胞存活率并减少细胞污染的机会。使用这种最先进的流式细胞仪和细胞分选仪将对UA研究人员和学生产生直接和巨大的影响。由于流式细胞术分析细胞类型，并进一步分选它们的基础上，他们的属性是绝对的先决条件，为执行高质量的生命科学和生物医学研究，FACSAria将推动UA？最近的健康研究计划，提高教师的研究能力，在新成立的生物医学工程系，并帮助更多的人在校园获得初步结果，以寻求国家科学基金会或国家卫生研究院的资金。