MRI: Acquisition of a Fluorescence Activated Cell Sorter for Biomedical & Bioscience Research and Training at University of Arkansas

1337265 jin：该提案寻求获得一种新的荧光激活细胞分选器（FACS） FACSAria III，以升级多用户共享的FACS，该FACS已服务于阿肯色大学（UA）整个校园超过18年。要求的新一代FACSAria III能够分选四种不同类型的细胞并同时分析九种荧光标记物。其分析和分选速度分别为75000个事件/秒和25000个细胞/秒，纯度为98%。这种快速分选能力将显著提高细胞存活率，减少细胞污染的机会。获得这种最先进的流式细胞仪和细胞分选器将对UA的研究人员和学生产生直接和巨大的影响。流式细胞术分析细胞类型并根据其性质对其进行进一步分类是进行高质量生命科学和生物医学研究的绝对先决条件，FACSAria将推动UA？在新成立的生物医学工程系，提高教师的研究能力，并帮助校园里更多的个人获得初步成果，以寻求国家科学基金会或国家卫生研究院的资助。

1337265JinThis proposal seeks to acquire a new fluorescence activated cell sorter (FACS), FACSAria III,to upgrade a multiuser-shared FACS that has been serving the entire campus of the University of Arkansas (UA) for more than 18 years. The requested new generation FACSAria III is capable of sorting four different types of cells and analyzing nine fluorescence markers simultaneously. Its rates of analysis and sorting are 75,000 events/second and 25,000 cells/second, respectively, with 98% of purity. This rapid sorting capability will significantly improve cell survival rate and reduce the chances of cell contamination. Access to this state-of-the-art flow cytometer and cell sorter will have an immediate and tremendous impact on UA researchers and students. Since flow cytometric analysis of cell types and further sorting them based on their properties are absolute pre-requisites for performing high-quality life science and biomedical research, FACSAria will propel UA?s recent health research initiative, boost faculty research capabilities in the newly-formed biomedical engineering department, and help more individuals on campus obtain preliminary results to seek NSF or NIH funding.