MRI: Acquisition of a Fluorescence Activated Cell Sorter for Biomedical & Bioscience Research and Training at University of Arkansas

1337265金这项提案寻求获得一种新的荧光激活细胞分类器(FACS)FACSAria III，以升级已为阿肯色大学(UA)整个校园服务了18年以上的多用户共享FACS。所要求的新一代FACSAria III能够对四种不同类型的细胞进行分选，并同时分析九种荧光标记。其分析和分选速度分别为75,000个事件/秒和25,000个细胞/秒，纯度为98%。这种快速分选能力将显著提高细胞存活率，减少细胞污染的机会。使用这种最先进的流式细胞仪和细胞分选仪将对UA的研究人员和学生产生直接和巨大的影响。由于对细胞类型进行流式细胞仪分析并根据其特性进行进一步分类是进行高质量生命科学和生物医学研究的绝对先决条件，FACSAria将推动加州大学S最近的健康研究倡议，提升新成立的生物医学工程系的教职员工研究能力，并帮助校园内更多的人获得初步结果，以寻求国家科学基金会或美国国立卫生研究院的资助。

1337265JinThis proposal seeks to acquire a new fluorescence activated cell sorter (FACS), FACSAria III,to upgrade a multiuser-shared FACS that has been serving the entire campus of the University of Arkansas (UA) for more than 18 years. The requested new generation FACSAria III is capable of sorting four different types of cells and analyzing nine fluorescence markers simultaneously. Its rates of analysis and sorting are 75,000 events/second and 25,000 cells/second, respectively, with 98% of purity. This rapid sorting capability will significantly improve cell survival rate and reduce the chances of cell contamination. Access to this state-of-the-art flow cytometer and cell sorter will have an immediate and tremendous impact on UA researchers and students. Since flow cytometric analysis of cell types and further sorting them based on their properties are absolute pre-requisites for performing high-quality life science and biomedical research, FACSAria will propel UA?s recent health research initiative, boost faculty research capabilities in the newly-formed biomedical engineering department, and help more individuals on campus obtain preliminary results to seek NSF or NIH funding.