CAREER: The Troponin Switch for Muscle Contraction

职业：肌钙蛋白开关促进肌肉收缩

• 批准号: 9985315
• 负责人: Taylor Allen
• 金额: $ 49.35万
• 依托单位: Oberlin College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9985315&HistoricalAwards=false
• 关键词: CAREER; Troponin; Switch; Muscle; Contraction

Support is requested through the CAREER program to merge two goals through collaborative curricular activities that fully engage undergraduate inquiry-based examinations of cell physiology and in the design and execution of experiments on the regulatory mechanism in striated muscle. The proposed research addresses two fundamental questions: 1) through what interactions within the troponin complex and between the complex and actin-tropomyosin does the troponin switch operate and 2) to what extent and by what mechanisms does TnI modulate the calcium sensitivity of force development, but also calcium-dependent process in general. The proposed experimentation draws not only on molecular, biological, and general techniques but also on thermodynamic and kinetic reasoning, quantitative skills and mechanical analysis.

支持要求通过职业计划，通过合作课程活动，充分参与本科调查为基础的细胞生理学考试，并在横纹肌的调节机制的实验的设计和执行合并两个目标。 拟议的研究解决了两个基本问题：1）肌钙蛋白复合物内以及复合物与肌动蛋白-原肌球蛋白之间的相互作用是肌钙蛋白开关起作用的，以及2）TnI在多大程度上以及通过何种机制调节力发展的钙敏感性，以及一般的钙依赖性过程。 拟议的实验不仅借鉴了分子、生物和通用技术，还借鉴了热力学和动力学推理、定量技能和力学分析。