Biomicromechanics of Stress-Assisted In-vitro Engineered Skin and Wound Remodeling

应力辅助体外工程皮肤和伤口重塑的生物微力学

• 批准号: 9988693
• 负责人: Ellen Arruda
• 金额: $ 8万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-15 至 2002-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9988693&HistoricalAwards=false
• 关键词: Biomicromechanics; Stress; Assisted; vitro; Engineered

9988693An environmental chamber for stress-assisted in vitro engineering of skin tissue will be built. Three dimensional natural collagen scaffolds will be immersed in a continuously replenished nutrient and seeded with fibroblast cells. This will be subjected to a sinusoidal loading at constant maximum load for at least 20 weeks. A similar collagen scaffold assay will be immersed in the same environment but not subjected to cyclic loads and deformations. The samples will be monitored periodically during growth to determine the effects of stress assistance on collagen density, anisotropy collagen moduli, fibroblast phenotype and fiber number density. The biomicromechanical constitutive modeling of collagen growth and remodeling will be pursues. The various known and speculated mechanisms driving collagen remodeling to HTS formation will be incorporated into the model as discussed. An anisotropic continuum model of collagen growth will be built using the experimental results to characterize the evolving network stiffness and anisotropy in relation to collagen growth.

9988693建立应力辅助皮肤组织体外工程环境舱。将三维天然胶原支架浸入持续补充的营养物中，并接种成纤维细胞。这将在恒定最大载荷下承受正弦载荷至少20周。将类似的胶原支架试验浸入相同的环境中，但不经受循环载荷和变形。在生长过程中定期监测样品，以确定应力辅助对胶原密度、各向异性胶原模量、成纤维细胞表型和纤维数密度的影响。胶原生长和重塑的生物细观力学本构模型是今后研究的方向。如所讨论的，将驱动胶原重塑以形成HTS的各种已知和推测的机制并入模型中。胶原蛋白生长的各向异性连续模型将使用实验结果来表征与胶原蛋白生长相关的不断变化的网络刚度和各向异性。