Biomicromechanics of Stress-Assisted In-vitro Engineered Skin and Wound Remodeling

9988693将建立一个应力辅助皮肤组织体外工程的环境室。三维天然胶原支架将被浸泡在持续补充的营养中，并种植成纤维细胞。它将在恒定的最大载荷下承受至少20周的正弦载荷。类似的胶原支架实验将浸泡在相同的环境中，但不会受到循环载荷和变形的影响。这些样本将在生长过程中定期监测，以确定应力辅助对胶原密度、各向异性胶原弹性系数、成纤维细胞表型和纤维数密度的影响。胶原蛋白生长和重塑的生物细观力学本构模型将是今后的研究方向。各种已知和推测的机制驱动胶原重塑到HTS的形成将被纳入到模型中讨论。利用实验结果建立胶原生长的各向异性连续模型，以表征与胶原生长相关的演化网络硬度和各向异性。

9988693An environmental chamber for stress-assisted in vitro engineering of skin tissue will be built. Three dimensional natural collagen scaffolds will be immersed in a continuously replenished nutrient and seeded with fibroblast cells. This will be subjected to a sinusoidal loading at constant maximum load for at least 20 weeks. A similar collagen scaffold assay will be immersed in the same environment but not subjected to cyclic loads and deformations. The samples will be monitored periodically during growth to determine the effects of stress assistance on collagen density, anisotropy collagen moduli, fibroblast phenotype and fiber number density. The biomicromechanical constitutive modeling of collagen growth and remodeling will be pursues. The various known and speculated mechanisms driving collagen remodeling to HTS formation will be incorporated into the model as discussed. An anisotropic continuum model of collagen growth will be built using the experimental results to characterize the evolving network stiffness and anisotropy in relation to collagen growth.