Biomicromechanics of Stress-Assisted In-vitro Engineered Skin and Wound Remodeling

9988693建立应力辅助皮肤组织体外工程环境室。三维天然胶原蛋白支架将浸泡在不断补充的营养物质中，并播种成纤维细胞。这将受到正弦加载在恒定的最大负荷至少20周。类似的胶原支架试验将浸泡在相同的环境中，但不受循环载荷和变形。在生长过程中，将对样品进行定期监测，以确定应力辅助对胶原密度、各向异性胶原模量、成纤维细胞表型和纤维数量密度的影响。对胶原生长和重塑的生物微力学本构模型进行了研究。各种已知的和推测的驱动胶原重塑到HTS形成的机制将被纳入模型。利用实验结果建立胶原生长的各向异性连续模型，以表征与胶原生长相关的网络刚度和各向异性的演变。

9988693An environmental chamber for stress-assisted in vitro engineering of skin tissue will be built. Three dimensional natural collagen scaffolds will be immersed in a continuously replenished nutrient and seeded with fibroblast cells. This will be subjected to a sinusoidal loading at constant maximum load for at least 20 weeks. A similar collagen scaffold assay will be immersed in the same environment but not subjected to cyclic loads and deformations. The samples will be monitored periodically during growth to determine the effects of stress assistance on collagen density, anisotropy collagen moduli, fibroblast phenotype and fiber number density. The biomicromechanical constitutive modeling of collagen growth and remodeling will be pursues. The various known and speculated mechanisms driving collagen remodeling to HTS formation will be incorporated into the model as discussed. An anisotropic continuum model of collagen growth will be built using the experimental results to characterize the evolving network stiffness and anisotropy in relation to collagen growth.