Strukturbestimmung eines Protein-RNA Komplexes mittels Kernspinresonanz und Kleinwinkelstreuung

人尖端相关蛋白（TAP）是信使核糖核酸（mRNA）的转运因子，介导其转录后从细胞核释放到细胞质中。在被类人猿D型逆转录病毒感染后，宿主TAP与组成性转运元件（CTE）复合物，通过形成TAP/CTE RNA复合物，将病毒mRNA释放到宿主细胞质中。虽然TAP的几个区域的结构已经通过晶体学研究解决了，但CTE RNA部分的结构以及它们相互作用的结构基础是未知的。在这个项目中，我打算将小角散射（SAS）与核磁共振（NMR）相结合，以获得缺乏的结构信息，这对于了解病毒在感染的人类细胞中的增殖具有重要的科学意义。除了科学和医学方面的兴趣外，该项目还将使我获得有关核磁共振和SAS技术的方法和实践知识，当这些技术协同使用时，非常有希望阐明TAP/CTE RNA和其他多聚体复合物的结构基础。

Human Tip associated protein (TAP) is a transport factor for messenger ribonucleic acids (mRNA), mediating their release from the cell nucleus into the cytoplasm after transcription. Upon infection by simian type D retroviruses, host TAP is complexed with a constitutive transport element (CTE) to allow release of viral mRNA into the host cytoplasm via formation of a TAP/CTE RNA complex. Whereas structures for the several regions of the TAP have been resolved by crystallographic studies, the structure of the CTE RNA part as well as the structural basis of their interaction is unknown. In this project, I intend to combine small angle scattering (SAS) with nuclear magnetic resonance (NMR) in order to obtain lacking structural information, which is of major scientific interest for understanding virus proliferation in infected human cells. Besides its scientific and medical interest, the project will allow me to acquire both methodological and practical know-how concerning the NMR as well as the SAS techniques, which, when used in concert, are very promising to shed light on the structural basis of the TAP/CTE RNA and other multimeric complexes.