Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia

该项目的目的是利用现代分子生物学和基因技术研究溶剂型梭状芽胞杆菌的代谢工程。这项跨学科建议的主要目标是从基因上改变乙酰丁酸梭菌的代谢。该建议描述了四个活性领域：(1)通过假定的抑制子SolR对Sol基因的调节进行详细研究；(2)对SporOA和ORF(OrfY)基因产物的功能进行分子分析，这两个基因产物都可能在Sol基因的表达中发挥重要作用；(3)利用cDNA阵列分析基因表达的模式；以及(4)进一步开发反义RNA作为代谢工程工具。

The objective of the project is to investigate the metabolic engineering of solventogenic Clostridia using modern molecular biology and genetic techniques. The primary goal of this interdisciplinary proposal is to genetically alter the metabolism of Clostridium acetobutylicum. The proposal describes four areas of activity; (1) a detailed examination of the regulation of the sol locus genes through a putative repressor, SolR; (2) a molecular analysis of the function of sporOA and of ORF (orfY) gene products, both of which are likely to be important in the expression of the sol locus genes; (3) an analysis of patterns of gene expression using cDNA arrays; and (4) the further development of antisense RNA as a metabolic engineering tool.