Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia

该项目的目的是利用现代分子生物学和遗传技术研究产溶剂梭菌的代谢工程。 这个跨学科的建议的主要目标是遗传改变丙酮丁醇梭菌的代谢。 该建议描述了四个活动领域：（1）通过假定的阻遏物SolR详细检查sol基因座基因的调节;（2）sporOA和ORF（orfY）基因产物的功能的分子分析，这两者在sol基因座基因的表达中可能是重要的;（3）使用cDNA阵列分析基因表达模式;（4）反义RNA作为代谢工程工具的进一步发展。

The objective of the project is to investigate the metabolic engineering of solventogenic Clostridia using modern molecular biology and genetic techniques. The primary goal of this interdisciplinary proposal is to genetically alter the metabolism of Clostridium acetobutylicum. The proposal describes four areas of activity; (1) a detailed examination of the regulation of the sol locus genes through a putative repressor, SolR; (2) a molecular analysis of the function of sporOA and of ORF (orfY) gene products, both of which are likely to be important in the expression of the sol locus genes; (3) an analysis of patterns of gene expression using cDNA arrays; and (4) the further development of antisense RNA as a metabolic engineering tool.