RoL: EAGER: DESYN-C3: Using synthetic energy-harvesting materials at the cell surface to reduce low potential ferredoxins within the cytosol for metabolic applications

RoL：EAGER：DESYN-C3：在细胞表面使用合成能量收集材料来减少细胞质内的低电位铁氧还蛋白，用于代谢应用

• 批准号: 1843556
• 负责人: George Bennett
• 金额: $ 30万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1843556&HistoricalAwards=false
• 关键词: RoL; EAGER; DESYN; C3; Using

Cells utilize many chemical sources of energy. Photosynthetic cells can use sunlight to create chemical bonds that store energy. That energy is used to shuttle electrons around the cell, driving reactions. Despite that, cells cannot utilize external sources of electrical energy directly. If they could utilize electricity as a power source, that would greatly reduce the burden on the cell to create energy on site, freeing it to be directed to more productive activities. This project is an attempt to create a cellular interface that allows that to happen. The external power source will be electricity generated by solar panels. The electricity will drive the production of an amino acid. Graduate students and undergraduates will be trained in advanced bioelectronics research techniques. The goal of this project is to build a modular cell-material interface that couples external electrical energy sources to the reduction of ferredoxins. The material side of the interface will enable attachment to a range of energy harvesting devices. The cell side will facilitate the transport of low potential electrons across the cell membrane to a ferredoxin, which will then be used to enhance the production of a reduced metabolite, the amino acid cysteine. The first objective is to engineer efficient electron transfer across the membrane. A high-throughput selection will be used to couple the growth of Escherichia coli to the electron cycling efficiency of low potential ferredoxin (Fd) electron carriers. The second objective is to build a biocompatible material (an organic electrode) that functions as a cell-surface coupling module. Diffusible mediators will be incorporated into the electrode and the effectiveness of organic phospholipids in mediating long-range electron transfer will be examined. The final objective is to use biological-organic hybrids to increase metabolic pathway yields and characterize electron transfer efficiencies. The interfaces arising from this project will be modular, and potentially useful in powering natural and synthetic cell processes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

细胞利用多种化学能源。光合作用细胞可以利用阳光产生化学键来储存能量。这种能量被用来在细胞内穿梭电子，驱动反应。尽管如此，电池不能直接利用外部电能。如果他们能够利用电力作为能源，这将极大地减轻电池在现场产生能量的负担，使其能够被释放出来，用于更具生产力的活动。这个项目是一种尝试，试图创造一个允许这种情况发生的蜂窝接口。外部电源将是太阳能电池板产生的电力。电力将驱动一种氨基酸的生产。研究生和本科生将接受先进生物电子学研究技术的培训。该项目的目标是建立一个模块化的电池-材料接口，将外部电能来源与铁氧还蛋白的减少相结合。该接口的材料侧将能够连接到一系列能量收集设备。细胞一侧将促进低电势电子通过细胞膜传输到铁氧还蛋白，然后铁氧还蛋白将被用来促进还原代谢物-氨基酸半胱氨酸的产生。第一个目标是设计有效的跨膜电子转移。高通量选择将用于将大肠杆菌的生长与低电位铁还蛋白(FD)电子载体的电子循环效率结合起来。第二个目标是建造一种生物兼容材料(有机电极)，作为细胞-表面耦合模块。可扩散的介质将被加入到电极中，并将检验有机磷脂在调节远程电子转移方面的有效性。最终的目标是使用生物-有机杂化来增加代谢途径的产量和表征电子转移效率。该项目产生的接口将是模块化的，并可能在为天然和合成电池过程提供动力方面有用。该奖项反映了NSF的法定使命，并已通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Recombination of 2Fe-2S Ferredoxins Reveals Differences in the Inheritance of Thermostability and Midpoint Potential

• DOI: 10.1021/acssynbio.0c00303
• 发表时间: 2020-12-18
• 期刊: ACS SYNTHETIC BIOLOGY
• 影响因子: 4.7
• 作者: Campbell, Ian J.;Kahanda, Dimithree;Silberg, Jonathan J.
• 通讯作者: Silberg, Jonathan J.