Collaborative Research: Genomic Approaches to Metabolic Engineering of Solventogenic Clostridia
合作研究:产溶剂梭菌代谢工程的基因组方法
基本信息
- 批准号:0418289
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-09-01 至 2009-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Papoutsakis0418289The aims of this proposal are to use DNA arrays and a "systems biology" approach for the analysis and generation of asporogenous, solvent producing strains suitable for continuous or fed-batch bioprocessing, and for studying large cellular programs (sporulation) and phenotypes (solventogenesis and degeneration). Genomic-scale DNA arrays will be used to capture the global picture of cellular transcriptional programs. These studies will be complemented by fermentation and microscopic studies to capture the differentiation process of the various strains. An important aim of both fundamental and practical significance is to determine the gene or genes on the pSOL1-megaplasmid responsible for inhibiting sporulation upon pSOL1 loss. An independent strategy will be also pursued in order to generate asporogenous but solventogenic strains. A third aim is to develop a critical genetic tool for both metabolic engineering (ME) synthesis and functional-genomic analysis, namely that of an efficient recombination system for chromosomal integration and gene deletion based on advanced genetic mechanisms such as the group II intron technology. A fourth aim is to develop strains with new and desirable characteristics in terms of solvent production and efficient bioprocessing, by employing several genetic and ME tools. This project provides unique opportunities for student education and training in the fast growing fields of genomics and ME in an advanced interdisciplinary research environment.
本提案的目的是使用DNA阵列和“系统生物学”方法来分析和生成适合连续或分批补料生物处理的产孢子、溶剂菌株,并用于研究大型细胞程序(孢子形成)和表型(溶剂发生和退化)。基因组规模的DNA阵列将用于捕捉细胞转录程序的全局图像。这些研究将辅以发酵和显微镜研究,以捕捉各种菌株的分化过程。确定pSOL1巨质粒上在pSOL1丢失时抑制产孢的基因是一个重要的基础和现实意义。还将采取一项独立的战略,以产生产菌种但具有溶剂性的菌株。第三个目标是开发一种用于代谢工程(ME)合成和功能基因组分析的关键遗传工具,即基于先进遗传机制(如II组内含子技术)的染色体整合和基因缺失的有效重组系统。第四个目标是通过使用几种遗传和ME工具,在溶剂生产和高效生物处理方面开发具有新的和理想特性的菌株。该项目为快速发展的基因组学和ME领域的学生提供了独特的教育和培训机会,并提供了先进的跨学科研究环境。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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George Bennett其他文献
Comparison of droperidol, haloperidol and prochlorperazine as postoperative anti-emetics
- DOI:
10.1007/bf03013781 - 发表时间:
1979-03-01 - 期刊:
- 影响因子:3.300
- 作者:
Edward A. Loeser;George Bennett;Theodore H. Stanley;Richard Machin - 通讯作者:
Richard Machin
Estimation of groundwater recharge from groundwater level fluctuations and baseflow rates around Mount Meru, Tanzania
坦桑尼亚梅鲁山附近地下水位波动和基流速率对地下水补给的估算
- DOI:
10.1016/j.gsd.2024.101133 - 发表时间:
2024-05-01 - 期刊:
- 影响因子:5.600
- 作者:
George Bennett;Ceven Shemsanga;Matthieu Kervyn;Kristine Walraevens - 通讯作者:
Kristine Walraevens
Assessment of spatial and temporal variability of groundwater level in the aquifer system on the flanks of Mount Meru, Northern Tanzania
坦桑尼亚北部梅鲁山侧翼含水层系统地下水位时空变异性评估
- DOI:
10.1016/j.ejrh.2022.101212 - 发表时间:
2022-12-01 - 期刊:
- 影响因子:5.000
- 作者:
George Bennett;Marc Van Camp;Ceven Shemsanga;Matthieu Kervyn;Kristine Walraevens - 通讯作者:
Kristine Walraevens
Analysis of methods used to validate remote sensing and GIS-based groundwater potential maps in the last two decades: A review
- DOI:
10.1016/j.geogeo.2023.100245 - 发表时间:
2023-11 - 期刊:
- 影响因子:0
- 作者:
George Bennett - 通讯作者:
George Bennett
George Bennett的其他文献
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{{ truncateString('George Bennett', 18)}}的其他基金
RoL: EAGER: DESYN-C3: Using synthetic energy-harvesting materials at the cell surface to reduce low potential ferredoxins within the cytosol for metabolic applications
RoL:EAGER:DESYN-C3:在细胞表面使用合成能量收集材料来减少细胞质内的低电位铁氧还蛋白,用于代谢应用
- 批准号:
1843556 - 财政年份:2018
- 资助金额:
-- - 项目类别:
Standard Grant
REU Site: Interdisciplinary Program in Multi-scale Biological Networks
REU 站点:多尺度生物网络跨学科项目
- 批准号:
1262296 - 财政年份:2013
- 资助金额:
-- - 项目类别:
Continuing Grant
Generation of biofuels from abundant non-digestible oilseed components
利用丰富的不易消化的油籽成分生产生物燃料
- 批准号:
1033552 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Standard Grant
REU Site: Interdisciplinary Program in Multi-scale Biomolecular Networks
REU 网站:多尺度生物分子网络跨学科项目
- 批准号:
1004853 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Continuing Grant
Transforming Organizations to Maximize Engineering Student Learning across the Texas A&M University System
变革组织以最大限度地提高德克萨斯州工程学生的学习能力
- 批准号:
0806022 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Standard Grant
LC/MS to Enhance Project-Based Laboratories
LC/MS 增强项目实验室
- 批准号:
0126135 - 财政年份:2002
- 资助金额:
-- - 项目类别:
Standard Grant
Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
合作研究:产溶剂梭菌的遗传分析和代谢工程
- 批准号:
0001288 - 财政年份:2000
- 资助金额:
-- - 项目类别:
Continuing grant
Green Chemistry: Characterization of Optically-Active Compounds as a Means for Introducing Chemistry, Nursing, and Non-Science Majors to Environmentally-Benign Laboratory Methods
绿色化学:光学活性化合物的表征作为向化学、护理和非科学专业介绍环境友好的实验室方法的手段
- 批准号:
9952602 - 财政年份:2000
- 资助金额:
-- - 项目类别:
Standard Grant
Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
合作研究:产溶剂梭菌的遗传分析和代谢工程
- 批准号:
9604562 - 财政年份:1996
- 资助金额:
-- - 项目类别:
Continuing grant
Houghton College -- Connection to the Internet
霍顿学院——连接互联网
- 批准号:
9412975 - 财政年份:1994
- 资助金额:
-- - 项目类别:
Standard Grant
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