Dissertation Research: Phylogenetic Relationships and Pollination of Acridocarpus and Other Old World Malpighiaceae.

论文研究：Acridocarpus 和其他旧世界金虎尾科的系统发育关系和授粉。

• 批准号: 0073299
• 负责人: Donald Pfister
• 金额: $ 1万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2002-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0073299&HistoricalAwards=false
• 关键词: Dissertation; Research; Phylogenetic; Relationships; Pollination

0073299Baum, Donoghue, and Davis Malpighiaceae are a family of flowering plants that are an important element of forests and savannas in the Old and New World tropics. New World Malpighiaceae are pollinated by specialized oil-collecting bees (anthophorid females) and exhibit a highly conserved floral morphology despite tremendous diversity in fruit morphology and habit. These oil-collecting bees are absent from the Old World. Some Old World clades, like Acridocarpus, are presumed to be derived from New World lineages and they exhibit a combination of features associated with oil-bee pollination and with "buzz" pollination. These groups provide an opportunity to examine the consequences of the loss of specialized pollinators. The goals of this study by graduate student Charles Davis, under the direction of Drs. David Baum and Michael Donoghue, are (1) to infer the phylogenetic relationships within Malpighiaceae using chloroplast and nuclear DNA sequences, (2) to infer the phylogeny or genealogy of Acridocarpus species in order to examine patterns of floral morphological evolution associated with pollinator shifts, and (3) to document the pollination of Acridocarpus in African habitats. Preliminary molecular data indicate that Old World genera of Malpighiaceae belong to several separate lineages, suggesting that morphologies associated with buzz pollination have evolved more than once independently. However, several areas of the phylogeny remain poorly resolved. Furthermore, several additional Old World lineages need to be added to existing datasets. Analyses of Acridocarpus using ribosomal DNA sequences reveal two major clades, one including species endemic to Madagascar and New Caledonia and the other consisting of species distributed across Africa. Oil glands appear to be entirely absent in species from Madagascar and New Caledonia, but appear in some African species. Fieldwork in Africa indicates that species of Acridocarpus are pollinated by large xylocopine bees that buzz the anthers, and the floral glands produce sugars rather than oils. Resolving the phylogeny of Malpighiaceae with additional chloroplast DNA sequences, along with sequences of a nuclear phytochrome gene, will clarify the origin and relationships of the Old World species. Further phylogenetic work will allow an evaluation of whether Old World species with similar morphologies have evolved independently, and whether such floral evolution is associated with the loss of oil-collecting pollinators. This study will shed light on the patterns and mechanisms of morphological change following a shift in pollination system.

[00:73 . 299] baum、Donoghue和Davis是开花植物科，是新旧热带地区森林和稀树草原的重要组成部分。新世界malpiighiaceae是由专门的采油蜜蜂（雌蜂）授粉的，尽管果实形态和习性存在巨大差异，但花形态却高度保守。这些采油蜜蜂在旧大陆是不存在的。一些旧大陆的分支，如Acridocarpus，被认为是来自新世界的谱系，它们表现出与油蜂授粉和“嗡嗡”授粉相关的特征组合。这些群体为研究专业传粉媒介丧失的后果提供了机会。这项研究的目标是研究生查尔斯·戴维斯，在博士的指导下。David Baum和Michael Donoghue的研究工作是：(1)利用叶绿体和细胞核DNA序列推断malpiighiaceae植物的系统发育关系；(2)推断Acridocarpus物种的系统发育或谱系，以研究与传粉者转移相关的花形态进化模式；(3)记录非洲栖息地中Acridocarpus的授粉情况。初步的分子数据表明，旧大陆Malpighiaceae属属于几个独立的谱系，这表明与嗡嗡传粉相关的形态已经独立进化了不止一次。然而，系统发育的几个领域仍然没有得到很好的解决。此外，需要将几个额外的旧世界血统添加到现有的数据集中。利用核糖体DNA序列对Acridocarpus进行分析，揭示了两个主要分支，一个包括马达加斯加和新喀里多尼亚特有的物种，另一个包括分布在非洲各地的物种。在马达加斯加和新喀里多尼亚的物种中似乎完全没有油腺，但在一些非洲物种中出现。在非洲的田野调查表明，各种Acridocarpus是由大型木碱蜜蜂授粉的，它们会嗡嗡作响花药，花腺产生的是糖而不是油。利用叶绿体DNA序列和核光敏色素基因序列来解决malpiighiaceae的系统发育问题，将澄清旧大陆物种的起源和关系。进一步的系统发育工作将有助于评估具有相似形态的旧大陆物种是否独立进化，以及这种花的进化是否与采油传粉媒介的丧失有关。本研究将揭示传粉系统转变后植物形态变化的模式和机制。