Duplicate Genes and Plant Phylogeny: Phytochromes and the Rooting of Seed Plants, Angiosperms, and Eudicots

重复基因和植物系统发育：光敏色素和种子植物、被子植物和真双子叶植物的生根

• 批准号: 9806937
• 负责人: Donald Pfister
• 金额: $ 18.65万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 2001-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9806937&HistoricalAwards=false
• 关键词: Duplicate; Genes; Plant; Phylogeny; Phytochromes

Donoghue DEB 9806937 Major evolutionary questions concerning the root of the phylogenetic tree of seed plants, of flowering plants, and a major lineage within flowering plants (the eudicots) have received considerable attention from evolutionary biologists, but have resisted convincing resolution. New approaches are, therefore, needed. The PIs propose a new strategy in which they will analyze a series of gene duplications in the phytochrome family of nuclear genes from members of each group to help solve this problem. Duplicate gene rooting relies on simultaneous phylogenetic analysis of genes that resulted from a gene duplication event that occurred along the branch subtending the plant group of interest. This procedure yields an unrooted gene network wherein two gene trees should be connected by a single branch, and the best rooted species tree is the one that is congruent with the two gene trees when the network is rooted along the connecting branch. Phytochrome genes are particularly well-suited for this approach because major duplications in the gene family apparently have occurred along the branches leading to each of the three main plant groups that we are interested in. Moreover, our preliminary analyses reveal a high level of agreement among duplicate gene trees, ample nucleotide variation, and considerable support for a number of critical branching events. We therefore are confident that this approach will provide insight into early branching events for each of the phylogenies in question. Additionally, these studies will elucidate the evolution of the phytochrome gene family in plants, and will highlight and elaborate the duplicate gene rooting procedure, which we hope will be of use in solving other difficult rooting problems.

多诺霍DEB 9806937 关于种子植物、显花植物和显花植物（真双子叶植物）系统发生树的根的主要进化问题已经受到进化生物学家的相当大的关注，但一直没有令人信服的解决方案。 因此，需要采取新的办法。 PI提出了一种新的策略，他们将分析来自每个群体成员的光敏色素核基因家族中的一系列基因重复，以帮助解决这个问题。 重复基因生根依赖于由基因重复事件引起的基因的同时系统发育分析，所述基因重复事件发生在对着感兴趣的植物组的分支的沿着。 该过程产生无根基因网络，其中两个基因树应该由单个分支连接，并且最佳有根物种树是当网络沿着连接的分支有根时与两个基因树全等的物种树。 光敏色素基因特别适合这种方法，因为基因家族中的主要重复显然发生在我们感兴趣的三个主要植物类群的分支上。 此外，我们的初步分析揭示了高水平的协议之间的重复基因树，丰富的核苷酸变异，以及相当多的关键分支事件的支持。 因此，我们有信心，这种方法将提供深入了解早期分支事件的每一个问题的基因。 此外，这些研究将阐明植物光敏色素基因家族的进化，并将突出和阐述重复基因生根过程，我们希望这将有助于解决其他困难的生根问题。