Identification of genes as molecular markers to understand and assess adaptation of Douglas-fir to drought stress and climatic change

鉴定基因作为分子标记，以了解和评估花旗松对干旱胁迫和气候变化的适应

• 批准号: 141907419
• 负责人: Professor Dr. Ingo Ensminger
• 金额: --
• 依托单位: Department of Biology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/141907419?language=en
• 关键词: Identification; genes; molecular; markers; understand

Climate acts as selective force on the genetic and physiological growth response of trees and thereby leads to an adaptation to local climate conditions. Our project aims at understanding the diversity of drought responses amongst tree provenances from environmentally different habitats. This will be achieved by linking phenotypic variation (as expressed in e.g. gene expression, growth responses or isotopic composition) with allelic variation in candidate genes using an association genetics approach. For this purpose, intraspecific differences amongst various provenances of Douglas-fir will be studied to assess the diversity of responses to drought. Through this approach we will be able to understand adaptability, vulnerability or resistance of Douglas-fir provenances to future climatic conditions. This project complements projects 2, 3 and 4 outlined in the general research concept, which aim to understand the diversity of drought responses of Douglas-fir on different spatial and temporal scales, by providing genetic and molecular mechanisms underlying the physiological and phenotypic levels of the drought response of Douglas-fir.

气候对树木的遗传和生理生长反应起着选择性的作用，从而导致树木对当地气候条件的适应。我们的项目旨在了解来自不同环境栖息地的树木种源对干旱反应的多样性。这将通过使用关联遗传学方法将表型变异（如基因表达、生长反应或同位素组成）与候选基因的等位基因变异联系起来来实现。为此，我们将研究不同种源道格拉斯冷杉的种内差异，以评估其对干旱反应的多样性。通过这种方法，我们将能够了解道格拉斯冷杉种源对未来气候条件的适应性、脆弱性或抵抗力。本项目补充了总体研究概念中概述的项目2、项目3和项目4，旨在通过提供道格拉斯冷杉干旱响应生理和表型水平的遗传和分子机制，了解道格拉斯冷杉在不同时空尺度上干旱响应的多样性。

项目成果

Suitability of Illumina deep mRNA sequencing for reliable gene expression profiling in a non-model conifer species (Pseudotsuga menziesii)

Illumina 深度 mRNA 测序对非模式针叶树物种（Pseudotsuga menziesii）进行可靠基因表达谱分析的适用性

• DOI: 10.1007/s11295-013-0656-2
• 发表时间: 2013
• 期刊: Tree Genetics & Genomes
• 影响因子: 2.4
• 作者: Hess M;Wildhagen H;Ensminger I
• 通讯作者: Ensminger I