Kinetics and structural analysis of Ebony, a glial protein in the visual system of Drosophila

果蝇视觉系统中的胶质蛋白 Ebony 的动力学和结构分析

• 批准号: 146781222
• 负责人: Professor Dr. Bernhard T. Hovemann
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Zellbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/146781222?language=en
• 关键词: Kinetics; structural; analysis; Ebony; glial

In the visual system of arthropods histamine (HA) is the neurotransmitter for stimulus transmission from retina photoreceptor neurons to downstream postsynaptic neurons. We propose a recycling pathway for this neurotransmitter in Drosophila melanogaster (D. melanogaster) in which the two enzymes Ebony and Tan together with yet to be identified transporters hold key functions: HA from the synaptic cleft is fused to β-alanine by Ebony in the surrounding epithelial glia for inactivation. After transfer into photoreceptor neurons, β-alanyl-histamine or carcinine (CA) is hydrolyzed by Tan thereby replenishing the HA pool for vesicle loading. This working model strongly depends among others on the cata-lytic efficiency of Ebony. To verify the proposed recycling pathway with a key function of Ebony in neurotransmitter inactivation, it is essential to determine its reaction kinetics. Furthermore, enzyme structure will be solved to understand how specific recruitment of β-alanine and peptide bond formation can be performed in concert with fast HA transport into epithelial glia. As a prerequisite new expression systems and purification strategies will be employed that overcome the hitherto limited capability in preparing pure and active Ebony protein.

在节肢动物的视觉系统中，组胺（HA）是神经递质，用于刺激从视网膜光感受器神经元到下游突触后神经元的传播。我们提出了果蝇中该神经递质的回收途径（D. melanogaster），其中两个酶Ebony和Tan以及尚未确定的转运蛋白具有关键功能：HA中的HA来自合成裂的HA由周围的ebony Intaine Intaine Intaverial Epepepelelial clia融合到β-紫plas脉中。转移到光感受器神经元中后，通过棕褐色水解了β-丙基因或癌（CA），从而对HA池补充蔬菜载荷。这种工作模型在很大程度上取决于乌木的cata宽度效率。为了验证乌木在神经递质失活中的关键功能验证所提出的回收途径，必须确定其反应动力学。此外，将解决酶结构，以了解如何在快速的HA转运到上皮胶质中进行特定的β-丙氨酸和胡椒键的募集。作为先决条件，将采用新的表达系统和纯化策略，以克服迄今为止在准备纯净和活跃的乌木蛋白方面有限的能力。

项目成果

Endogenous ciliary neurotrophic factor modulates anxiety and depressive-like behavior

• DOI: 10.1016/j.bbr.2012.01.020
• 发表时间: 2012-04
• 期刊: Behavioural Brain Research
• 影响因子: 2.7
• 作者: Isabella Peruga;S. Hartwig;D. Merkler;J. Thöne;B. Hovemann;G. Juckel;R. Gold;R. Linker