Upgrade and Modernization of the Integrated Imaging Center

综合影像中心的升级和现代化

• 批准号: 0099706
• 负责人: J.Michael McCaffery
• 金额: $ 6.97万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-15 至 2003-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0099706&HistoricalAwards=false
• 关键词: Upgrade; Modernization; Integrated; Imaging; Center

This award provides support for acquisition of an ultramicrotome with cryostage and a glass knife maker to be placed in the Integrated Imaging Center located in the Johns Hopkins University Biology Department. The Integrated Imaging Center includes both light and electron microscopy equipment and thus provides university faculty and students convenient access to conventional and advanced techniques in both types of microscopy. The major focus of the Center is the utilization of fluorescent and electron dense probes for the purpose of investigating cellular/subcellular structure and function. In recent years, the development and refinement of new techniques that use antibodies to localize specific proteins within cells has become an essential component of any comprehensive study of subcellular structure and function. Typically, the antibodies used are chemically conjugated to fluorescent or electron dense moiety to facilitate visualization by microscopy. This complements and extends results obtained by other approaches. The Center offers both undergraduate and graduate courses in microscopy (as well as regular workshops) as a means of providingtraining and exposure to the latest, most advanced microscopy techniques to students and to faculty. These courses emphasize the integration of microscopic techniques with the use of other cell biological research tools. The new equipment will replace older existing equipment that can no longer function with the precision required for modern cell biology research. Among the projects that will make use of the equipment are studies of the endocytosis pathways of yeast and insect cells, analysis of mitochondrial dynamics in yeast, and investigation of the role of golgins in structure and function of the golgi apparatus.

该奖项将为约翰霍普金斯大学生物系集成成像中心的低温低温显微组和玻璃刀制造商提供支持。综合成像中心包括光学和电子显微镜设备，从而为大学教师和学生提供方便的访问传统和先进的技术在这两种类型的显微镜。该中心的主要重点是利用荧光和电子密度探针来研究细胞/亚细胞的结构和功能。近年来，利用抗体定位细胞内特定蛋白质的新技术的发展和完善已成为亚细胞结构和功能综合研究的重要组成部分。通常，使用的抗体是化学偶联到荧光或电子密集的片段，以方便显微镜观察。这是对其他方法得到的结果的补充和扩展。该中心提供显微镜的本科和研究生课程（以及定期研讨会），为学生和教师提供最新，最先进的显微镜技术的培训和接触。这些课程强调显微技术与其他细胞生物学研究工具的结合。新设备将取代现有的旧设备，这些设备已不能满足现代细胞生物学研究所需的精度。将利用该设备的项目包括酵母和昆虫细胞的内吞途径的研究，酵母线粒体动力学的分析，以及高尔基体结构和功能中高尔基蛋白的作用的研究。