SBIR Phase I: Thermostable Phage DNA Polymerases: Improved Tools for Genomics Research

SBIR 第一阶段：耐热噬菌体 DNA 聚合酶：基因组学研究的改进工具

• 批准号: 0109756
• 负责人: Thomas Schoenfeld
• 金额: $ 9.91万
• 依托单位: LUCIGEN CORPORATION
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109756&HistoricalAwards=false
• 关键词: SBIR; Phase; Thermostable; Phage; DNA

0109756SchoenfeldThis Small Business Innovation Research (SBIR) Phase I project will develop new thermostable bacteriophage and archaeaviral DNA polymerases for use as improved reagents for DNA amplification, sequence analysis and single nucleotide polymorphism (SNP) detection. This will be accomplished by direct isolation of thermophilic bacteriophage and archaeaviral genomes from hot springs, construction of expression libraries and screening for novel thermostable DNA polymerase activities. The proposed approach is expected to be significantly more rapid and comprehensive than traditional approaches that have been used for enzyme discovery. These traditional approaches rely on the limiting intermediate step of culturing a microbe and its cognate virus. In contrast, the proposed approach on this project will allow screening of all expressible viral DNA polymerases present, including those from unculturable phage that is believed to predominate in the environment.The primary commercial application of this project will be in the marketing of novel thermostable DNA polymerases to organizations involved in genomics research for use as improved reagents for specific molecular biology methods..

0109756Schoenfeld 这个小型企业创新研究 (SBIR) 第一阶段项目将开发新的耐热噬菌体和古病毒 DNA 聚合酶，用作 DNA 扩增、序列分析和单核苷酸多态性 (SNP) 检测的改进试剂。 这将通过从温泉中直接分离嗜热噬菌体和古病毒基因组、构建表达文库以及筛选新型热稳定性 DNA 聚合酶活性来实现。 所提出的方法预计将比用于酶发现的传统方法更加快速和全面。 这些传统方法依赖于培养微生物及其同源病毒的有限中间步骤。相比之下，该项目提出的方法将允许筛选存在的所有可表达的病毒DNA聚合酶，包括来自被认为在环境中占主导地位的不可培养噬菌体的聚合酶。该项目的主要商业应用将是向参与基因组学研究的组织推销新型热稳定DNA聚合酶，用作特定分子生物学方法的改进试剂。