SBIR Phase II: Thermostable Phage DNA Polymerases: Improved Tools for Genomics Research

这个小企业创新研究二期项目将开发新的DNA聚合酶试剂，用于当前和正在开发的DNA诊断程序。该方法是开发嗜热噬菌体DNA复制酶来代替目前使用的DNA修复酶。在第一阶段的研究中证明了这种方法的可行性。第二阶段的后续项目将扩展第一阶段使用的方法，以分离更多的活性物质，对其进行表征，并将其开发为各种扩增平台的试剂。这个项目的商业应用将在一些使用DNA分子分析的市场上。这些领域包括生物医学研究、医学测试、基因鉴定测试、公共卫生和农业。

This Small Business Innovation Research Phase II project will develop novel DNA polymerase reagents for use in current and developing DNA diagnostic procedures. The approach is to develop thermophilic phage DNA replicases in place of the currently used DNA repair enzymes. The feasibility of this approach was demonstrated during Phase I research. This follow on Phase II project will extend the methods used in Phase I to isolate additional activities, characterize them and develop them as reagents for various amplification platforms. The commercial applications of this project will be in a number of markets that use molecular analysis of DNA. They include the areas of biomedical research, medical testing, genetic identity testing, public health and agriculture.