RUI - Functional Characterization of a Nematode Gene Related to the Class II bHLH Protein, ABF-1

RUI - 与 II 类 bHLH 蛋白 ABF-1 相关的线虫基因的功能表征

• 批准号: 0111069
• 负责人: Craig Vierra
• 金额: $ 13.94万
• 依托单位: University of the Pacific
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0111069&HistoricalAwards=false
• 关键词: RUI; Functional; Characterization; Nematode; Gene

Basic helix-loop-helix proteins are important transcriptional regulators of cellular proliferation and differentiation in organisms ranging from invertebrates to vertebrates. Recently, a new gene closely related to the mammalian basic helix-loop-helix protein activated B-cell factor 1 (ABF-1), designated CeABF-1, was cloned from the nematode Caenorhabditis elegans. The objectives of the research include 1) investigating the function of the CeABF-1 gene; 2) elucidating the transcriptional properties of the CeABF-1 protein; 3) identifying the target gene(s) regulated by CeABF-1. To accomplish these specific goals, gene function and expression studies will be performed to delineate the role of CeABF-1 in the nematode. The functional studies will integrate a broad spectrum of genetic and biochemical techniques, including RNA microinjections and the development of transgenic animals for expression analyses. The transcriptional properties and target gene(s) regulated by CeABF-1 will be analyzed using DNA transfections, electrophoretic-mobility shift assays and DNA microanalysis. This project will allow for the comparison of HLH gene function in both nematodes and humans and identify conserved molecular pathways important for cellular processes. The results of these studies will help to elucidate the evolutionary as well as the biological relationship between the nematode and human ABF-1 protein. Helix-loop-helix (HLH) proteins are important transcriptional regulators of cellular proliferation and differentiation in organisms ranging from invertebrates to vertebrates. The importance of HLH proteins as potent mediators of immune system function and muscle development has been well established. The significance of these studies will allow for the determination of the biological function of the nematode Caenorhabditis elegans HLH gene, designated CeABF-1, and will provide data that will help to elucidate the molecular mechanisms that govern cell-restricted eukaryotic gene expression. This project will enhance the knowledge of target gene(s) controlled by the protein CeABF-1, and provide an understanding of the ancestral relationship between the worm CeABF-1 and human homologue, activated B-cell factor 1 (ABF-1).

从无脊椎动物到脊椎动物，基本的螺旋-环-螺旋蛋白是细胞增殖和分化的重要转录调节因子。最近，一个与哺乳动物碱性螺旋-环-螺旋蛋白激活的b细胞因子1 （ABF-1）密切相关的新基因，被命名为CeABF-1，从线虫隐杆线虫中克隆。本研究的目的包括：1)研究CeABF-1基因的功能；2)阐明CeABF-1蛋白的转录特性；3)确定CeABF-1调控的靶基因。为了实现这些特定的目标，将进行基因功能和表达研究，以描述CeABF-1在线虫中的作用。功能研究将整合广泛的遗传和生化技术，包括RNA显微注射和用于表达分析的转基因动物的发展。CeABF-1调控的转录特性和靶基因将通过DNA转染、电泳迁移转移试验和DNA微分析进行分析。该项目将允许比较HLH基因在线虫和人类中的功能，并确定对细胞过程重要的保守分子途径。这些研究结果将有助于阐明线虫与人ABF-1蛋白之间的进化和生物学关系。从无脊椎动物到脊椎动物，螺旋-环-螺旋（HLH）蛋白是细胞增殖和分化的重要转录调节因子。HLH蛋白作为免疫系统功能和肌肉发育的有效介质的重要性已经得到了很好的证实。这些研究的意义将允许确定秀丽隐杆线虫HLH基因（指定为CeABF-1）的生物学功能，并将提供有助于阐明控制细胞限制性真核基因表达的分子机制的数据。该项目将增强对CeABF-1蛋白控制的靶基因的认识，并提供对蠕虫CeABF-1与人类同源物活化b细胞因子1 （ABF-1）的祖先关系的理解。