MRI: Acquisition of Mass Spectrometry Technology for Research and Training

MRI：获取质谱技术用于研究和培训

• 批准号: 1531417
• 负责人: Craig Vierra
• 金额: $ 57.91万
• 依托单位: University of the Pacific
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531417&HistoricalAwards=false
• 关键词: MRI; Acquisition; Mass; Spectrometry; Technology

An award is made to the University of the Pacific to do proteomics research through the acquisition of mass spectrometry technology. The research integrates comprehensive mentoring programs, workshops, and provides training for students pursuing careers in science and engineering, as well as health-related professions. The instrumentation will provide investigators with the opportunity to train students across a broad range of educational tiers, including high school, undergraduate and graduate student levels. By implementing curricular additions, hands-on proteomic activities in genetics and molecular biological technique courses will provide students with powerful opportunities to learn how to combine genomic and proteomic approaches to solve scientific questions. Additionally, outreach activities that target local high schools, will enable students to collect cobwebs from the environment with glass rods, wash the fibers with water, and learn how to use mass spectrometry to identify biomolecules in real-life materials. Investigators will also promote the engagement of underrepresented groups by encouraging economically disadvantaged and minority high school student participation through Project SEED (Scholarships for Education and Economic Development). The impact of the instrumentation on student learning and STEM education will be assessed by electronic pre- and post-assessment questionnaires. Results from the research will enhance scientific literacy for members in the community, highlighting important concepts in materials development as well as conservational biology. Activities of the research project are innovative and cutting-edge, involving expertise that spans biology, chemistry, biochemistry, bioengineering, and the health sciences. These scientific investigations will advance our knowledge on the molecular mechanisms that govern spider silk assembly, leading to new strategies for synthetic fiber production for industrial and commercial applications. It will also provide insight into the chemical properties of biopolymers resembling the active sites of proteins as well as the machinery governing cell movement and developmental processes in vertebrates. Additionally, it will identify novel factors that regulate protein secretion in eukaryotic cells, providing protein chemist with new approaches to make recombinant protein expression systems more robust. Lastly, they will elucidate constituents that modulate the metabolism of human toxins, identify pathogenic factors that contribute to human cancer formation, and provide answers to important evolutionary and environmental questions in biology. As a collective unit, these studies will be highly transformative for students, faculty, and the community, leading to the development of new biomaterials for engineering and nanotechnology, better methods for recombinant protein expression for academia and industry, increased comprehension with cellular migration mechanisms, and advances in knowledge to fundamental themes in evolution and conservation biology.

太平洋大学通过获得质谱技术进行蛋白质组学研究获得奖励。该研究整合了全面的指导计划，研讨会，并为追求科学和工程职业的学生提供培训，以及与健康相关的专业。该仪器将为研究人员提供培训各种教育层次的学生的机会，包括高中、本科和研究生。通过增加课程内容，遗传学和分子生物学技术课程中的蛋白质组学实践活动将为学生提供学习如何结合基因组学和蛋白质组学方法来解决科学问题的强大机会。此外，针对当地高中的外展活动将使学生能够用玻璃棒从环境中收集蜘蛛网，用水清洗纤维，并学习如何使用质谱法识别现实生活材料中的生物分子。调查人员还将通过SEED项目（教育和经济发展奖学金）鼓励经济弱势和少数族裔高中生参与，从而促进代表性不足群体的参与。仪器对学生学习和STEM教育的影响将通过评估前和评估后的电子问卷进行评估。这项研究的结果将提高社区成员的科学素养，突出材料发展和保护生物学的重要概念。该研究项目的活动具有创新性和尖端性，涉及生物学、化学、生物化学、生物工程和健康科学等领域的专业知识。这些科学研究将促进我们对控制蜘蛛丝组装的分子机制的了解，为工业和商业应用的合成纤维生产提供新的策略。它还将提供深入了解生物聚合物的化学性质，类似于蛋白质的活性位点，以及脊椎动物控制细胞运动和发育过程的机制。此外，它将确定调节真核细胞中蛋白质分泌的新因素，为蛋白质化学家提供新的方法，使重组蛋白表达系统更加强大。最后，他们将阐明调节人类毒素代谢的成分，确定导致人类癌症形成的致病因素，并为生物学中重要的进化和环境问题提供答案。作为一个集体单位，这些研究将对学生、教师和社区具有高度的变革意义，导致用于工程和纳米技术的新生物材料的开发，为学术界和工业界提供更好的重组蛋白表达方法，增加对细胞迁移机制的理解，并在进化和保护生物学的基本主题方面取得进展。