Atomic Scale Imaging Implemented Into Undergraduate Science Education

原子尺度成像应用于本科科学教育

• 批准号: 0126402
• 负责人: Maria Hepel
• 金额: $ 12.23万
• 依托单位: SUNY College at Potsdam
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0126402&HistoricalAwards=false
• 关键词: Atomic; Scale; Imaging; Implemented; Into

Interdisciplinary (99)In this project, four science departments (Chemistry, Biology, Physics, and Geology) at SUNY Potsdam are using Atomic Force Microscopy and Scanning Tunneling Microscopy (AFM/STM) to continue their ongoing efforts to incorporate advanced technologies into laboratory experiences for science majors. While examples of atomic imaging techniques in new textbooks have enhanced student's visualization of atoms and molecules, the lack of an AFM and appropriate related laboratory experiences has limited student learning in this area. Bridging the gap between coursework and the laboratory, a number of new experiments based on AFM/STM use are being developed, some of which are integrated across the curriculum. The ability to engage students in experiments using AFM/STM is creating possibilities for designing innovative courses and is advancing undergraduate research. This equipment enables students in chemistry and biology classes to (1) measure the forces between molecules, including antigen/antibody interactions (V.W. Jones, J.R. Kenseth, M.D. Porter, C.L. Mosher, and E. Henderson, "Microminiaturized Immunoassays Using Atomic Force Microscopy and Compositionally Patterned Antigen Arrays," Anal. Chem., 1998, 70, 1233-1241), (2) explore phospholipid and drug interactions (T.H. Ha, C.H. Kim, J.S. Park, and K. Kim, "Interaction of Indolicidin with Model Lipid Bilayer: Quartz Crystal Microbalance and Atomic Force Microscopy Study," Langmuir, 2000, 16, 871-875), (3) examine structure of polymer films (D. Raghavan, M. VanLandingham, X. Gu, and T. Nguyen, "Characterization of Heterogeneous Regions in Polymer Systems Using Tapping Mode and Force Mode Atomic Force Microscopy," Langmuir, 2000, 16, 9448-9459), (4) observe pitting corrosion and to study the effect of inhibitors on metal corrosion, imaging the surface of nanoelectrocatalytic materials used for methanol oxidation, (5) examine microporosity and mesoporosity in different carbon materials, (6) observe adherence of bacteria to surfaces, (7) examine the structure of biological molecules at very high resolution, and (8) study the viral infection process. Biology and chemistry students are now able to study the actions of DNA binding proteins and ligands, including restriction enzyme mapping and gene localization (H.B. Sun, L. Qian, and H. Yokota, "Detection of Abasic Sites on Individual DNA Molecules Using Atomic Force Microscopy," Anal. Chem., 2001,73, 2229-2232). The data generated through AFM analysis of these processes are then compared with those generated through traditional methods, and demonstrate the evolution of modern chemistry and molecular biology, while providing the students with a glimpse of the future direction of the science.

跨学科 (99) 在该项目中，纽约州立大学波茨坦分校的四个科学系（化学、生物学、物理和地质学）正在使用原子力显微镜和扫描隧道显微镜 (AFM/STM) 继续努力，将先进技术融入科学专业的实验室经验中。虽然新教科书中原子成像技术的例子增强了学生对原子和分子的可视化，但缺乏原子力显微镜和适当的相关实验室经验限制了学生在该领域的学习。为了弥合课程作业和实验室之间的差距，我们正在开发一些基于 AFM/STM 使用的新实验，其中一些实验已整合到整个课程中。让学生参与使用 AFM/STM 进行实验的能力为设计创新课程创造了可能性，并正在推进本科生研究。该设备使化学和生物学课程的学生能够 (1) 测量分子之间的作用力，包括抗原/抗体相互作用（V.W. Jones、J.R. Kenseth、M.D. Porter、C.L. Mosher 和 E. Henderson，“使用原子力显微镜和组合图案化抗原阵列的微型免疫测定”，Anal. Chem.，1998 年，70， 1233-1241），（2）探索磷脂和药物相互作用（T.H. Ha、C.H. Kim、J.S. Park 和 K. Kim，“Indolicidin 与模型脂质双层的相互作用：石英晶体微天平和原子力显微镜研究”Langmuir，2000, 16, 871-875），（3）检查聚合物膜的结构（D. Raghavan、M. VanLandingham、X. Gu 和 T. Nguyen，“使用轻敲模式和力模式原子力显微镜表征聚合物系统中的异质区域”，Langmuir，2000, 16, 9448-9459），(4) 观察点蚀并研究抑制剂对金属腐蚀的影响，对表面进行成像 用于甲醇氧化的纳米电催化材料，（5）检查不同碳材料的微孔和中孔，（6）观察细菌对表面的粘附，（7）以非常高分辨率检查生物分子的结构，以及（8）研究病毒感染过程。生物学和化学专业的学生现在能够研究 DNA 结合蛋白和配体的作用，包括限制性内切酶图谱和基因定位（H.B. Sun、L. Qian 和 H. Yokota，“Detection of Abasic Sites on individual DNA Molecules using Atomic Force Microscopy”，Anal. Chem.，2001,73, 2229-2232）。然后将通过 AFM 分析这些过程生成的数据与通过传统方法生成的数据进行比较，展示现代化学和分子生物学的演变，同时让学生了解科学的未来方向。