Atomic Scale Imaging Implemented Into Undergraduate Science Education

跨学科（99）在这个项目中，纽约州立大学波茨坦的四个科学系（化学、生物学、物理学和地质学）正在使用原子力显微镜和扫描隧道显微镜（AFM/STM）继续他们正在进行的努力，将先进技术融入科学专业的实验室体验。虽然新教科书中原子成像技术的例子增强了学生对原子和分子的可视化，但缺乏原子力显微镜和适当的相关实验室经验限制了学生在这一领域的学习。弥合课程和实验室之间的差距，一些新的实验基于AFM/STM的使用正在开发中，其中一些是跨课程集成。利用AFM/STM让学生参与实验的能力为设计创新课程创造了可能性，并推动了本科生的研究。该设备使化学和生物课的学生能够（1）测量分子之间的力，包括抗原/抗体相互作用（V.W.琼斯，J.R. Kenseth，医学博士Porter，C.L. Mosher和E.亨德森，“使用原子力显微镜和组成图案化抗原阵列的微型化免疫测定”，分析。化学成分：1998，70，1233-1241），（2）探索磷脂和药物相互作用（T.H. CH Kim，J.S. Park和K. Kim，“Interaction of Indolicidin with Model Lipid Bilayer：Quartz Crystal Microbalance and Atomic Force Microscopy Study，”Langmuir，2000，16，871-875），（3）检查聚合物膜的结构（D. M. Raghavan VanLandingham，X. Gu和T. Nguyen，“Characterization of Heterogeneous Regions in Polymer Systems Using Tapping Mode and Force Mode Atomic Force Microscopy，”Langmuir，2000，16，9448-9459），（4）观察点蚀并研究抑制剂对金属腐蚀的影响，成像用于甲醇氧化的纳米电催化材料的表面，（5）检查不同碳材料中的微孔和中孔，（6）观察细菌对表面的粘附，（7）以非常高的分辨率检查生物分子的结构，以及（8）研究病毒感染过程。生物学和化学的学生现在能够研究DNA结合蛋白和配体的作用，包括限制性内切酶图谱和基因定位（H. B。孙湖，加-地Qian和H. Yokota，“使用原子力显微镜检测单个DNA分子上的非碱性位点”，Anal。化学成分：2001，73，2229-2232）。然后将通过AFM分析这些过程产生的数据与通过传统方法产生的数据进行比较，并展示现代化学和分子生物学的演变，同时为学生提供科学未来方向的一瞥。

Interdisciplinary (99)In this project, four science departments (Chemistry, Biology, Physics, and Geology) at SUNY Potsdam are using Atomic Force Microscopy and Scanning Tunneling Microscopy (AFM/STM) to continue their ongoing efforts to incorporate advanced technologies into laboratory experiences for science majors. While examples of atomic imaging techniques in new textbooks have enhanced student's visualization of atoms and molecules, the lack of an AFM and appropriate related laboratory experiences has limited student learning in this area. Bridging the gap between coursework and the laboratory, a number of new experiments based on AFM/STM use are being developed, some of which are integrated across the curriculum. The ability to engage students in experiments using AFM/STM is creating possibilities for designing innovative courses and is advancing undergraduate research. This equipment enables students in chemistry and biology classes to (1) measure the forces between molecules, including antigen/antibody interactions (V.W. Jones, J.R. Kenseth, M.D. Porter, C.L. Mosher, and E. Henderson, "Microminiaturized Immunoassays Using Atomic Force Microscopy and Compositionally Patterned Antigen Arrays," Anal. Chem., 1998, 70, 1233-1241), (2) explore phospholipid and drug interactions (T.H. Ha, C.H. Kim, J.S. Park, and K. Kim, "Interaction of Indolicidin with Model Lipid Bilayer: Quartz Crystal Microbalance and Atomic Force Microscopy Study," Langmuir, 2000, 16, 871-875), (3) examine structure of polymer films (D. Raghavan, M. VanLandingham, X. Gu, and T. Nguyen, "Characterization of Heterogeneous Regions in Polymer Systems Using Tapping Mode and Force Mode Atomic Force Microscopy," Langmuir, 2000, 16, 9448-9459), (4) observe pitting corrosion and to study the effect of inhibitors on metal corrosion, imaging the surface of nanoelectrocatalytic materials used for methanol oxidation, (5) examine microporosity and mesoporosity in different carbon materials, (6) observe adherence of bacteria to surfaces, (7) examine the structure of biological molecules at very high resolution, and (8) study the viral infection process. Biology and chemistry students are now able to study the actions of DNA binding proteins and ligands, including restriction enzyme mapping and gene localization (H.B. Sun, L. Qian, and H. Yokota, "Detection of Abasic Sites on Individual DNA Molecules Using Atomic Force Microscopy," Anal. Chem., 2001,73, 2229-2232). The data generated through AFM analysis of these processes are then compared with those generated through traditional methods, and demonstrate the evolution of modern chemistry and molecular biology, while providing the students with a glimpse of the future direction of the science.