U.S.-France Cooperative Research: Investigation of Polyploid Genome Evolution

0128936温德尔美国-法国在植物基因组研究方面的合作涉及爱荷华州州立大学和法国雷恩第一大学的学生和教师研究人员。乔纳森·F. Wendel和Malika Ainouche将分别领导美国和法国的合作项目。 他们将在两个互补的模型系统中研究多倍体的形成和新表达模式的进化，一个来自棉花属，另一个来自草属米草属。 利用来自两个属的材料和天然种群的多样性，将在新合成的和旧的天然多倍体中研究基因表达。 将评价多倍体形成后立即发生的变化以及在进化时间尺度上发生的变化。 研究人员还将使用cDNA-AFLP筛选来确定基因沉默的程度和动态，以识别，测序和验证。与其二倍体亲本相比，将检查多倍体中表达和新表达的组织特异性分配的重复基因，其中初始筛选表明沉默或新表达。 爱荷华州集团为这次合作带来了棉花属天然和合成多倍体成员的专业知识。 法国对来自草属米草的多倍体系统的研究和调查补充了这一点。该奖项代表了美国方面向NSF和法国国家科学研究中心（CNRS）提出的联合建议。 NSF将承担美国研究人员和研究生的旅费和生活费。 国家科学研究中心将支持法国参与者访问美国。该合作将促进对高等植物中进化新基因表达的理解，并改善棉花农业。

0128936WendelThis three-year award for U.S.-France collaboration in plant genome research involves students and faculty researchers at Iowa State University and University of Rennes 1, Rennes, France. Jonathan F. Wendel and Malika Ainouche will lead the collaborative program in the US and France respectively. They will study polyploid formation and evolution of novel expression patterns in two complementary model systems, one from the cotton genus Gossypium and the other from the grass genus Spartina. Using a diversity of materials and natural populations from both genera, gene expression will be studied in newly synthesized and older, natural polyploids. Changes that occur immediately after polyploid formation as well as those that arise on an evolutionary time-scale will be evaluated. The researchers will also determine the extent and dynamics of gene silencing using a cDNA-AFLP screen to identify, sequence and verify. Tissue specific partitioning of expression and novel expression in polyploids, compared to their diploid parents, will be examined for duplicated genes where initial screens suggest silencing or novel expression. The Iowa State group brings to this collaboration expertise in natural and synthetic polyploid members of the cotton genus Gossypium. French studies and investigations of the polyploid system from the grass genus Spartina complement this. This award represents the US side of joint proposals to the NSF and the French National Center for Scientific Research (CNRS). NSF will cover travel funds and living expenses of the US investigators and graduate students. The CNRS will support visits to US of the French participants. The collaboration will advance understanding of the evolution novel gene expression in higher plants and improvements in cotton agriculture.