Microbial Genome Sequencing: Phages of Agronomic Bacteria: A Student-Based Genomics Approach

将建立一个以学生为基础的项目，建立一个噬菌体基因组数据库，这些噬菌体感染农学上重要的细菌，特别是农业病原体。最初的细菌宿主将是丁香假单胞菌，它会引起许多重要的植物疾病。经过一段时间的开发，该项目将构成噬菌体基因组学高级本科/研究生初级课程的实验室核心，学生团队将采用噬菌体，确定和注释其完整的基因组序列，并在一整年的监督研究和讲座课程活动中进行标准的物理和生理分析。除了教育目的之外，噬菌体基因组数据库将成为解决植物细菌宿主背景下噬菌体进化问题和噬菌体对植物-细菌相互作用影响的资源，并探索噬菌体鸡尾酒在植物急性或慢性疾病生物防治应用中的可能用途。这是一个微生物基因组测序奖，由国家科学基金会和农业部未来农业和粮食系统倡议之间的合作活动资助。

A student-based program to establish a genomic database of bacteriophages that infect agronomically important bacteria, especially agricultural pathogens will be established. The initial bacterial host will be Pseudomonas syringae, which causes a number of important plant diseases. After a development period, the program will constitute the laboratory core of an advanced undergraduate/beginning graduate course in Phage Genomics, in which the teams of students will adopt a bacteriophage, determine and annotate its complete genome sequence, and conduct standard physical and physiological analyses during a full year of supervisedresearch and lecture course activity. In addition to the educational goals served by this effort, thephage genomics database will be a resource to address questions about bacteriophage evolutionin the context of plant bacterial hosts and the influence of phage on plant-bacteria interactions,and to probe the possible utility of phage cocktails in biocontrol applications against acute orchronic diseases of plants. This is a Microbial Genome Sequencing Award funded through a collaborative activity between the National Science Foundation and the Department of Agriculture Initiative for Future Agriculture and Food Systems.