Remorin-mediated signal transduction

Remorin介导的信号转导

• 批准号: 158026536
• 负责人: Professor Dr. Thomas Ott
• 金额: --
• 依托单位: Arbeitsgruppe Zellbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Independent Junior Research Groups
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/158026536?language=en
• 关键词: Remorin; mediated; signal; transduction

Plant microbe interactions require several receptors, cytosolic and nuclear proteins to act in concert to trigger molecular and morphological responses to approaching symbiotic or pathogenic bacteria and fungi. We recently identified a novel type of scaffold protein belonging to the remorin family in Medicago truncatula (MtSYMREM1) that is able to interact with at least three receptor-like kinases that are essential for root-nodule symbiosis. While we characterised roles of this protein during later stages of the interaction, its functions during host signal perception of rhizobial Nod Factors and early events of infection remains unknown. The first part of the project aims for the analysis of SYMREM1 protein dynamics with a focus on localisation and interactions with other proteins in its homologous background. In the second part, we will extend our current knowledge to non-legumes. Using Arabidopsis thaliana as a model plant we will characterise remorins from other subfamilies and take a global approach to identify signalling pathways that require presence of remorin proteins. Detailed biochemical, genetic and advanced microscopical analyses will provide valuable information to elucidate the functions of these proteins.

植物微生物相互作用需要几种受体、胞质和核蛋白协同作用，以触发对接近共生或致病细菌和真菌的分子和形态反应。我们最近发现了一种新型的支架蛋白，属于蒺藜苜蓿（MtSYMREM 1），能够与至少三个受体样激酶，是必不可少的根瘤共生家族。虽然我们在相互作用的后期表征了这种蛋白质的作用，但其在根瘤菌结瘤因子的宿主信号感知和感染的早期事件期间的功能仍然未知。该项目的第一部分旨在分析SYMREM 1蛋白质动力学，重点是在其同源背景中与其他蛋白质的定位和相互作用。在第二部分中，我们将把我们现有的知识扩展到非豆类。以拟南芥为模式植物，我们将从其他亚科中提取拟南芥素，并采取一种全局性的方法来识别需要拟南芥素蛋白存在的信号通路。详细的生物化学、遗传学和先进的显微镜分析将为阐明这些蛋白质的功能提供有价值的信息。