Symbiosis-dependent microbiome patterning in Lotus japonicus roots

莲子根中共生依赖的微生物组模式

• 批准号: 401841933
• 负责人: Professor Dr. Thomas Ott
• 金额: --
• 依托单位: Forschungsschwerpunkt Zellbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/401841933?language=en
• 关键词: Symbiosis; dependent; microbiome; patterning; Lotus

Sequencing a number of microbiomes from the legume Lotus japonicus provided great insights into the complexity of endophytes present in the rhizosphere and associated with the host root. However, our knowledge on spatio-temporal colonization patterns and any cross-talk in between these intercellularly colonizing bacteria and fungi is sparse as these information cannot be retrieved from global sequencing data. Furthermore, almost all genetic studies in legumes that assessed host genes for their function in controlling colonization by symbiotic microbes such as rhizobia and arbuscular mycorrhiza fungi (AMF) have been conducted by applying mono-association setups. However, the situation is fundamentally different in natural soils, where legume roots are colonized by multiple microbes simultaneously. Therefore, this proposal aims to increase complexity in the experimental setup by addressing the following aspects:1. Assessing a range of symbiosis mutants for their performance in natural soils. Based on our pilot experiments we will screen a collection of Lotus mutants for their ability to bypass regulatory networks that are currently considered to be indispensable for RNS. Strains colonizing these mutants will be identified. 2. Large-scale testing of mono-associations initially using 70 isolates from a bacterial core collection that was generated from Lotus japonicus roots. The most effective colonizers will be combined to a minimal synthetic community (minimal SynCom).3. Generation of marker strains within the minimal SynCom.4. Analysing distribution patterns of these marker strains within the host root and initial analysis of possible host factors controlling them.

对豆科植物百脉根中的许多微生物组进行测序，为了解根际中存在的以及与宿主根相关的内生菌的复杂性提供了深入的见解。然而，我们对时空定植模式以及这些细胞间定植细菌和真菌之间的任何串扰的了解很少，因为这些信息无法从全局测序数据中检索。此外，几乎所有评估宿主基因在控制根瘤菌和丛枝菌根真菌（AMF）等共生微生物定殖中的功能的豆类遗传研究都是通过应用单关联设置进行的。然而，在天然土壤中情况却截然不同，豆科植物的根部同时被多种微生物定殖。因此，该提案旨在通过解决以下几个方面来增加实验设置的复杂性：1． 评估一系列共生突变体在天然土壤中的表现。根据我们的试点实验，我们将筛选一组 Lotus 突变体，以确定它们绕过目前被认为是 RNS 不可或缺的监管网络的能力。将鉴定定殖这些突变体的菌株。 2. 最初使用从莲花根产生的细菌核心保藏中的 70 个分离株对单一关联进行大规模测试。最有效的殖民者将被组合成一个最小的合成社区（最小的SynCom）。3。 在最小 SynCom.4 内生成标记菌株。 分析这些标记菌株在寄主根内的分布模式，并对可能控制它们的寄主因素进行初步分析。