Structure and function of the contractile vacuoles of Chlamydomonas reinhardtii (Chlorophyta)

莱茵衣藻（绿藻门）收缩液泡的结构和功能

• 批准号: 158148367
• 负责人: Professor Dr. Burkhard Becker
• 金额: --
• 依托单位: Institut für Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/158148367?language=en
• 关键词: Structure; function; contractile; vacuoles; Chlamydomonas

Contractile vacuoles (CVs) are osmoregulatory organelles found in many unicellular organisms. Little is known about the function of CVs in general and especially in green algae. I have recently started to analyze the structure and function of CVs in the two chlorophyte algae Chlamydomonas reinhardtii and Scherffelia dubia, and the streptophyte Mesostigma viride. Although the three algae represent most likely three different invasions of the freshwater habitat, the structure and function of the CV in these genera show several similarities. Chlamydomonas is the only genetically tractable organism of these three algae. For this reason, I plan to use Chlamydomonas for the studies outlined in this grant proposal. The major focus of this work will be the isolation of CV mutants in Chlamydomonas using an insertional mutagenesis approach. We will then perform a functional analysis of the isolated mutants. In addition we will investigate the function of V-ATPase, vacuolar pyrophosphatase (V-PPase), and the single aquaporin present in the genome of Chlamydomonas. These proteins have been implicated in CV function in various systems including Chlamydomonas. I will investigate the function of these proteins using RNAi, GFP/antibody technology combined with light- and electron microscopy.

收缩泡（Contractile vacuoles，CV）是一种存在于许多单细胞生物中的细胞调节细胞器。关于CV的功能，特别是在绿色藻类中的CV的功能知之甚少。我最近开始分析的结构和功能的CV在两个绿藻Chlamyeliumreinhardtii和Scherffelia dubia，和链霉菌Mesostigma viride。虽然这三种藻类很可能代表了淡水栖息地的三种不同入侵，但这些属中CV的结构和功能显示出一些相似之处。披衣藻是这三种藻类中唯一遗传上易处理的生物。出于这个原因，我计划使用衣原体的研究概述在此拨款建议。这项工作的主要重点是使用插入诱变方法在衣原体中分离CV突变体。然后我们将对分离的突变体进行功能分析。此外，我们还将研究衣原体基因组中存在的V-ATP酶、液泡焦磷酸酶（V-PPase）和单个水通道蛋白的功能。这些蛋白质在包括衣原体在内的各种系统中与CV功能有关。我将使用RNAi，GFP/抗体技术结合光学和电子显微镜来研究这些蛋白质的功能。