Structure and function of the contractile vacuoles of Chlamydomonas reinhardtii (Chlorophyta)

莱茵衣藻（绿藻门）收缩液泡的结构和功能

• 批准号: 158148367
• 负责人: Professor Dr. Burkhard Becker
• 金额: --
• 依托单位: Institut für Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/158148367?language=en
• 关键词: Structure; function; contractile; vacuoles; Chlamydomonas

Contractile vacuoles (CVs) are osmoregulatory organelles found in many unicellular organisms. Little is known about the function of CVs in general and especially in green algae. I have recently started to analyze the structure and function of CVs in the two chlorophyte algae Chlamydomonas reinhardtii and Scherffelia dubia, and the streptophyte Mesostigma viride. Although the three algae represent most likely three different invasions of the freshwater habitat, the structure and function of the CV in these genera show several similarities. Chlamydomonas is the only genetically tractable organism of these three algae. For this reason, I plan to use Chlamydomonas for the studies outlined in this grant proposal. The major focus of this work will be the isolation of CV mutants in Chlamydomonas using an insertional mutagenesis approach. We will then perform a functional analysis of the isolated mutants. In addition we will investigate the function of V-ATPase, vacuolar pyrophosphatase (V-PPase), and the single aquaporin present in the genome of Chlamydomonas. These proteins have been implicated in CV function in various systems including Chlamydomonas. I will investigate the function of these proteins using RNAi, GFP/antibody technology combined with light- and electron microscopy.

收缩液泡（CVS）是在许多单细胞生物中发现的渗透调节细胞器。关于简历通常，尤其是绿藻的功能知之甚少。我最近开始分析CVS在两个叶绿素藻类衣原体Reinhardtii和Scherffelia dubia以及链球菌中层中的结构和功能。尽管三种藻类最有可能是淡水栖息地的三种不同入侵，但在这些属中，简历的结构和功能显示出几种相似之处。衣原体是这三种藻类的唯一遗传处理生物。因此，我计划将衣原体使用该赠款提案中概述的研究。这项工作的主要重点是使用插入诱变方法在衣原体中分离CV突变体。然后，我们将对孤立的突变体进行功能分析。此外，我们将研究V-ATPase，液泡焦距磷酸酶（V-PPase）和存在于衣原体基因组中的单一水通道蛋白的功能。这些蛋白质已与包括衣原体在内的各种系统中的CV功能有关。我将使用RNAI，GFP/抗体技术与光和电子显微镜结合研究这些蛋白质的功能。