Functional metatranscriptomics of Antarctic biological soil crust algae: Identification of key genes for the survival under the extreme conditions of Antarctica

南极生物土壤结皮藻类的功能宏转录组学：鉴定南极极端条件下生存的关键基因

• 批准号: 442927497
• 负责人: Professor Dr. Burkhard Becker
• 金额: --
• 依托单位: Institut für Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Infrastructure Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/442927497?language=en
• 关键词: Functional; metatranscriptomics; Antarctic; biological; soil

Terrestrial green algae are typical and abundant components of biological soil crusts (BSCs) in the Polar Regions. These communities form water-stable aggregates that have important ecological roles in primary production, nitrogen fixation, nutrient cycling, water retention and stabilization of soils. Although available data on green algae are generally very limited for the Arctic and Antarctica, their functional importance as ecosystem developers in nutrient poor environments is regarded as high. Recently, the biodiversity of algae and cyanobacteria has been determined by us for the first time using classical and molecular methods (Metatranscriptomics and Metabarcoding). In the new project, we want to use metatranscriptomes of Antarctic BSCs to determine the physiological status of the microalgae within the BSCs. To achieve this goal, we will assign the sequences within the metatranscriptomes to biological species (genera, families or other suitable categories). For functional analysis of the submetatranscriptomes we will compare (qualitatively and quantitatively) the sequences with several public databases (KOG; GEGG; GO) and the available transcriptomes. For further analysis, we will compare the submetatranscriptomes with transcriptomes obtained for cultures, of Antarctic Klebsormidium and Coccomyxa strains, under laboratory conditions (liquid culture/plate culture, desiccation, temperature stress and light stress). Several laboratory transcriptomes are available for Klebsormidium. For further comparison with the Arctic submetatranscriptomes we will establish transcriptomes in the laboratory from two additional Klebsormidium and two Coccomyxa strains isolated from the Arctic. The obtained data will allow for the first time to address the physiological status of polar algae in situ in their natural habitat at the time of sampling using molecular data. Furthermore, the comparison of the submetatranscriptomes for Klebsormidium and Coccomyxa will allow the identification of key genes for the survival within the Antarctic.

陆生绿藻是极地生物土壤结皮(BSCs)的典型组成部分。这些群落形成水稳定的团聚体，在初级生产、固氮、养分循环、保水和土壤稳定方面具有重要的生态作用。尽管关于北极洲和南极洲绿藻的现有数据通常非常有限，但它们作为营养不良环境中的生态系统开发者的功能重要性被认为是很高的。最近，我们首次使用经典和分子方法(元转录组学和元编码)确定了藻类和蓝藻的生物多样性。在这个新的项目中，我们希望利用南极BSCs的转录本来确定BSCs中微藻的生理状态。为了实现这一目标，我们将把元译本中的序列分配给生物物种(属、科或其他合适的类别)。对于亚转录本的功能分析，我们将与几个公共数据库(KOG；Gegg；Go)和可用的转录本进行比较(定性和定量)。为了进一步的分析，我们将在实验室条件下(液体培养/平板培养，干燥，温度胁迫和光照胁迫)将亚变录本与南极克雷伯氏菌和球孢子菌的培养获得的转录本进行比较。克雷伯氏杆菌有几种实验室转录本。为了进一步与北极的亚变种进行比较，我们将在实验室中建立另外两个克雷伯氏菌和两个从北极分离的球霉菌的转录本。所获得的数据将首次允许利用分子数据在采样时就地处理极地藻类在其自然栖息地的生理状态。此外，Klebsormidium和Coccomyxa的亚变态转录本的比较将有助于识别在南极生存的关键基因。