Investigations of the Mechanism of Vanadium Haloperoxidases

钒卤过氧化物酶机制的研究

• 批准号: 0213523
• 负责人: Alison Butler
• 金额: --
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0213523&HistoricalAwards=false
• 关键词: Investigations; Mechanism; Vanadium; Haloperoxidases

This award by the Inorganic, Bioinorganic and Organometallic Chemistry program supports research by Alison Butler, University of California Santa Barbara Department of Chemistry and Biochemistry. Butler is studying haloperoxidase enzymes, more specifically the vanadium bromoperoxidases found in several types of marine algae. The mechanisms of these enzymes and the biogenesis of halogenated terpene and acetogenin marine natural products will be explored. Using site-directed mutagenesis, Butler will determine the functional role of selected amino acid residues in the enzymes and prepare mutants suitable for in vitro reactivity. These mutants may eventually be used for the industrial synthesis of halogenated compounds suitable for medical applications.In addition to the fundamental bioinorganic chemistry and enzymology proposed, the work has broad implications for marine biology and pharmaceutical chemistry. Butler also trains a diverse group of students in an emerging interdisciplinary field

该奖项由无机、生物无机和有机金属化学项目颁发，支持加州大学圣巴巴拉分校化学与生物化学系艾莉森·巴特勒的研究。巴特勒正在研究卤素过氧化物酶，更具体地说，是几种海洋藻类中发现的钒溴过氧化物酶。本文将对这些酶的作用机制以及卤代萜烯和醋酸原等海洋天然产物的生物生成进行探讨。利用定点诱变，Butler将确定选定的氨基酸残基在酶中的功能作用，并制备适合体外反应的突变体。这些突变体可能最终用于适合医疗应用的卤化化合物的工业合成。除了提出基本的生物无机化学和酶学外，这项工作对海洋生物学和药物化学具有广泛的意义。巴特勒还在一个新兴的跨学科领域培养不同群体的学生