Microbial Iron Acquisition: Investigations of Amphiphilic Siderophores

微生物铁的获取：两亲性铁载体的研究

• 批准号: 1059067
• 负责人: Alison Butler
• 金额: $ 44.76万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1059067&HistoricalAwards=false
• 关键词: Microbial; Iron; Acquisition; Investigations; Amphiphilic

This award in the Chemistry of Life Processes (CLP) program supports work by Professor Alison Butler at the University of California, Santa Barbara to carry out fundamental studies on the process of iron acquisition by bacteria that is mediated by siderophores. Nearly all bacteria require iron to grow. Siderophores are low molecular mass chelating ligands produced by bacteria that coordinate Fe(III) with very high affinity and facilitate iron uptake by the bacterium. Marine bacteria often produce suites of amphiphilic siderophores that vary in the nature of the fatty acid tail. The interactions of the amphiphilic siderophores, their self-assembled structures and the bacteria will be probed to investigate whether the amphiphilic character of these siderophores confers a distinct molecular advantage for microbial iron acquisition.This project supports the education and training of graduate and undergraduate students in a highly interdisciplinary research project in a manner that will serve to broaden and deepen the level of research training. Some marine bacteria grow on petroleum hydrocarbons as their sole source of carbon and energy; yet before bacteria can begin to oxidize hydrocarbons, they must first acquire iron for growth and then for assimilation into the key enzymes. Thus understanding how oil-degrading bacteria acquire the essential nutrient, iron, would benefit efforts to enhance microbial hydrocarbon oxidation of environmental concern.

生命过程化学（CLP）计划中的这一奖项支持加州大学圣巴巴拉分校的Alison Butler教授的工作，以开展关于细菌铁吸收过程的基础研究，该过程由铁载体介导。几乎所有的细菌都需要铁来生长。 铁载体是由细菌产生的低分子量螯合配体，其以非常高的亲和力与Fe（III）配位并促进细菌对铁的吸收。 海洋细菌通常产生一套两亲性铁载体，其脂肪酸尾部的性质各不相同。 本研究将探讨两亲性铁载体及其自组装结构与细菌的相互作用，以研究这些铁载体的两亲性是否为微生物铁的获取提供了明显的分子优势。本项目支持研究生和本科生在高度跨学科研究项目中的教育和培训，以拓宽和深化研究培训水平。 有些海洋细菌以石油碳氢化合物为唯一的碳源和能量来源;然而，在细菌开始氧化碳氢化合物之前，它们必须首先获得铁来生长，然后同化成关键的酶。 因此，了解石油降解细菌如何获得必需的营养素铁，将有助于提高环境关注的微生物碳氢化合物氧化的努力。