Acquisition of a Quadrupole-Time-of-Flight Mass Spectrometer

购买四极杆飞行时间质谱仪

• 批准号: 0079773
• 负责人: Alison Butler
• 金额: $ 24.25万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0079773&HistoricalAwards=false
• 关键词: Acquisition; Quadrupole; Time; Flight; Mass

Mass spectrometry has recently become one of the most powerful techniques for structural characterization of biological molecules, including peptides, proteins and nucleic acids. Tandem mass spectrometry is particularly useful for sequencing these molecules, especially when only small amounts of material are available. The quadrupole time-of-flight tandem mass spectrometer will be used for routine fragmentation and unequivocal daughter fragment identification and characterization. Specific projects which will use this instrumentation include 1) investigations of the molecular mechanisms of iron acquisition by marine microorganisms in order to understand the role of iron in regulating the global carbon cycle and climate change; 2) the search for biological molecular markers of autism, 3) the synthesis and characterization of polymeric peptide adhesives with industrial and medical applications, 4) the development biological nanofabrication processes of new high performance composites and 5) investigations of amphiphilic peptides to control the cell response, particularly on biologically active material surfaces. The quadrupole time-of flight mass spectrometer will include a high pressure liquid chromatograph to separate compounds before analyzing their masses and fragmentation patterns. The mass spectrometer will consist of a quadrupole as the first mass analyzer, followed by a quadrupole collision cell and a time-of-flight detector as the second mass analyzer. The system will also be configured with nanospray and micro ion spray capabilities to allow for a range of flow rates and to permit the small amounts of biological samples to be analyzed. The system will use electrospray ionization (ESI) for soft ionization of proteins and peptides or atmospheric pressure chemical ionization (APCI) for analysis of polar organic molecules. The quadrupole time-of flight mass spectrometer will significantly enhancing the research infrastructure at the University of California, Santa Barbara by extending the mass range and sensitivity capabilities of the UCSB Mass Spectrometry Facility which is housed in the Department of Chemistry and Biochemistry. For example, this instrument provides the capability to elucidate the structures of peptides, proteins and nucleic acids, which is required by the research groups that will use this instrumentation. This mass spectrometer will also significantly enhance student training, by providing exposure to and training in modern structure determination methodologies, which will certainly be useful in the emerging areas of proteomics, genomics and bioinformatics.

质谱法近年来已成为生物分子结构表征最有力的技术之一，包括多肽、蛋白质和核酸。串联质谱法对这些分子的测序特别有用，特别是当只有少量的材料可用时。四极杆飞行时间串联质谱仪将用于常规碎片和明确的子碎片鉴定和表征。将使用该仪器的具体项目包括：1)研究海洋微生物获取铁的分子机制，以了解铁在调节全球碳循环和气候变化中的作用；2)寻找自闭症的生物分子标记，3)工业和医疗应用的聚合肽粘合剂的合成和表征，4)开发新型高性能复合材料的生物纳米制造工艺，5)研究控制细胞反应的两亲肽，特别是在生物活性材料表面。四极杆飞行时间质谱仪将包括一个高压液相色谱仪，在分析它们的质量和破碎模式之前分离化合物。质谱仪将由一个四极杆作为第一个质量分析器，随后是一个四极杆碰撞单元和一个飞行时间检测器作为第二个质量分析器。该系统还将配置纳米喷雾和微离子喷雾功能，以允许一定范围的流速，并允许少量生物样品进行分析。该系统将使用电喷雾电离（ESI）对蛋白质和多肽进行软电离，或使用大气压化学电离（APCI）对极性有机分子进行分析。四极杆飞行时间质谱仪将通过扩展位于化学和生物化学系的UCSB质谱设备的质量范围和灵敏度能力，显著增强加州大学圣巴巴拉分校的研究基础设施。例如，该仪器提供了阐明肽、蛋白质和核酸结构的能力，这是将使用该仪器的研究小组所需要的。这台质谱仪还将通过提供现代结构测定方法的接触和培训，显著加强学生的培训，这在蛋白质组学、基因组学和生物信息学等新兴领域肯定是有用的。