Mechanistic Investigations of Vanadium Haloperoxidases

钒卤过氧化物酶的机理研究

• 批准号: 0719553
• 负责人: Alison Butler
• 金额: $ 45.5万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0719553&HistoricalAwards=false
• 关键词: Mechanistic; Investigations; Vanadium; Haloperoxidases

This award in the Inorganic, Bioinorganic and Organometallic Chemistry program supports work by Professor Alison Butler at the University of California at Santa Barbara to probe reactions catalyzed by vanadium bromoperoxidases (V-BrPO) from species of marine red algae in the biosynthesis of brominated spiro--bicyclic sesquiterpene natural products, brominated fused-ring diterpene natural products, brominated acyclic monoterpene natural products, and bromofuranone natural products. The mechanism of action of these enzymes will be further investigated through bromine isotope fractionation studies. Many marine natural products and enzymes have important industrial applications in the specialty chemical, diagnostics, pharmaceutical and biotechnology industries. Mechanistic enzyme studies of V-BrPO can be used to form the basis of an economical, alternative and environmentally sound source of brominated marine natural products, as well as further elucidating the mechanism of halogenation. Elucidation of pathways for the biosynthesis of new compounds of biomedical and industrial importance is an anticipated impact. This project supports the education and training of graduate students, undergraduate students and postdoctoral fellows in a highly interdisciplinary research project in a manner that will serve to broaden and deepen the level of research training. The graduate students and PI participate in an extensive UCSB campus network providing undergraduate research training and K-12 outreach, across diverse populations.

无机、生物无机和有机化学项目的这一奖项支持了加州大学圣巴巴拉分校的Alison Butler教授的工作，以探测来自海洋红藻物种的钒溴过氧化物酶（V-BrPO）催化的反应，这些反应是溴化螺环-双环倍半萜天然产物、溴化稠环二萜天然产物、溴化无环单萜天然产物、和溴呋喃酮天然产物。 将通过溴同位素分馏研究进一步研究这些酶的作用机制。许多海洋天然产品和酶在特种化学品、诊断、制药和生物技术工业中具有重要的工业应用。V-BrPO的机理酶研究可用于形成溴化海洋天然产物的经济、替代和环境无害的来源的基础，以及进一步阐明卤化的机理。阐明具有生物医学和工业重要性的新化合物的生物合成途径是预期的影响。 该项目支持研究生、本科生和博士后研究员在高度跨学科研究项目中的教育和培训，其方式将有助于扩大和深化研究培训水平。研究生和PI参加广泛的UCSB校园网络，提供本科研究培训和K-12外展，在不同的人群。