Bioinorganic Chemistry of Catechols: Siderophores, Adhesive Proteins and Biomimetic Analogs

儿茶酚的生物无机化学：铁载体、粘附蛋白和仿生类似物

• 批准号: 1710761
• 负责人: Alison Butler
• 金额: $ 48万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1710761&HistoricalAwards=false
• 关键词: Bioinorganic; Chemistry; Catechols; Siderophores; Adhesive

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Professor Alison Butler from the University of California, Santa Barbara to investigate the properties of compounds called catechol-siderophores that are produced by bacteria. The compounds are similar to the molecules that make it possible for mussels to cling tenaciously to rocks in the ocean. This research examines the reasons for the recently discovered properties that allow mussels to cling even to slick, wet surfaces. This project may provide an understanding of the biological phenomenon and may lead to the design of compounds that can adhere even under water. The role of catechol-siderophores in iron acquisition by bacteria is also investigated. This research project provides broad multidisciplinary training for graduate and undergraduate students. Professor Butler and her students are involved in outreach workshops including, CAMP (California Alliance for Minority Participation), an NSF-funded program supporting minority undergraduate students in science, technology, engineering and mathematics fields, and SCOPE (Science as a Career Outreach Project Experiment), a program for graduate students to talk in local high schools about undergraduate and graduate education and the value of the sciences as a career choice.The research is investigates siderophores and analogs containing both catechol and amine functional groups that mimic the strong wet adhesive properties of mussel foot proteins. The overall goals of the first part of this proposal are to elucidate the synergistic role of the catechol and the amine functionalities in displacing the interfacial hydration layer that develops on mineral surfaces leading to robust adhesion. The approach is to design and investigate the surface adhesive properties of new biomimetic compounds through direct force measurements with a surface forces apparatus. The goals of the second part of this research are to investigate the biosynthesis, uptake, and iron(III) release mechanisms of acylated tetra L-serine, and tris-catechol siderophores through investigation of key fatty acid coenzyme A ligases, outer membrane receptor proteins, and esterases identified in the genomes of the producing bacteria.

有了这个奖项，化学部门的生命过程化学项目资助了加州大学圣巴巴拉分校的艾莉森·巴特勒教授，研究细菌产生的儿茶酚-铁载体化合物的性质。这些化合物类似于使贻贝能够在海洋中顽强地附着在岩石上的分子。这项研究探讨了最近发现的贻贝甚至可以附着在光滑潮湿的表面上的特性的原因。这个项目可以提供对生物现象的理解，并可能导致设计出即使在水下也能粘附的化合物。儿茶酚-铁载体在细菌获取铁中的作用也进行了研究。该研究项目为研究生和本科生提供广泛的多学科培训。巴特勒教授和她的学生参与了一些外展研讨会，包括CAMP（加州少数民族参与联盟），这是一个由美国国家科学基金会资助的项目，支持科学、技术、工程和数学领域的少数民族本科生，以及SCOPE（科学作为职业外展项目实验），这是一个让研究生在当地高中谈论本科和研究生教育以及科学作为职业选择的价值的项目。该研究调查了含有儿茶酚和胺官能团的铁载体和类似物，它们模拟了贻贝足蛋白质的强湿粘合特性。本提案第一部分的总体目标是阐明儿茶酚和胺功能在取代矿物表面上形成的界面水化层的协同作用，从而导致牢固的粘附。方法是设计和研究新的仿生化合物的表面粘附性能，通过直接力测量与表面力装置。本研究第二部分的目标是通过研究产菌基因组中鉴定的关键脂肪酸辅酶A连接酶、外膜受体蛋白和酯酶，研究酰基化四l -丝氨酸和三儿茶酚铁载体的生物合成、摄取和铁（III）释放机制。

项目成果

Precursor-directed biosynthesis of catechol compounds in Acinetobacter bouvetii DSM 14964

布韦不动杆菌中儿茶酚化合物的前体定向生物合成 DSM 14964

• DOI: 10.1039/d0cc04171h
• 发表时间: 2020
• 期刊: Chemical Communications
• 影响因子: 4.9
• 作者: Reitz, Zachary L.;Butler, Alison
• 通讯作者: Butler, Alison

Impact of Molecular Architecture and Adsorption Density on Adhesion of Mussel-Inspired Surface Primers with Catechol-Cation Synergy

• DOI: 10.1021/jacs.9b04337
• 发表时间: 2019-11-27
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Degen, George D.;Stow, Parker R.;Israelachvili, Jacob N.
• 通讯作者: Israelachvili, Jacob N.

Ambiguity of NRPS Structure Predictions: Four Bidentate Chelating Groups in the Siderophore Pacifibactin

• DOI: 10.1021/acs.jnatprod.8b01073
• 发表时间: 2019-04-01
• 期刊: JOURNAL OF NATURAL PRODUCTS
• 影响因子: 5.1
• 作者: Hardy, Clifford D.;Butler, Alison
• 通讯作者: Butler, Alison

Ruckerbactin Produced by Yersinia ruckeri YRB Is a Diastereomer of the Siderophore Trivanchrobactin Produced by Vibrio campbellii DS40M4

由鲁克氏耶尔森菌 YRB 产生的 Ruckerbactin 是由坎贝氏弧菌 DS40M4 产生的铁载体 Trivanchrobactin 的非对映异构体

• DOI: 10.1021/acs.jnatprod.1c01047
• 发表时间: 2021
• 期刊: Journal of Natural Products
• 影响因子: 5.1
• 作者: Thomsen, Emil;Reitz, Zachary L.;Stow, Parker R.;Dulaney, Kalana;Butler, Alison
• 通讯作者: Butler, Alison

A suite of asymmetric citrate siderophores isolated from a marine Shewanella species

• DOI: 10.1016/j.jinorgbio.2019.110736
• 发表时间: 2019-09-01
• 期刊: JOURNAL OF INORGANIC BIOCHEMISTRY
• 影响因子: 3.9
• 作者: Carmichael, Jeffrey R.;Zhou, Hongjun;Butler, Alison
• 通讯作者: Butler, Alison