Wechselwirkungen von polyphilen Molekülen mit Lipidmonolayern und Lipidvesikeln

多亲分子与脂质单层和脂质囊泡的相互作用

• 批准号: 161163068
• 负责人: Professor Dr. Alfred Blume
• 金额: --
• 依托单位: Arbeitsbereich Physikalische und Theoretische Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161163068?language=en
• 关键词: Wechselwirkungen; von; polyphilen; Molek; len

Goals of this project are investigations and elucidation of the interactions of low molecular weight and polymeric polyphilic molecules in aqueous solution with lipid model membranes and lipid monolayers. Low molecular weight polyphiles will have T- and X-like structures and will be synthesized in the group of C. Tschierske (TP 2) while the polymeric polyphiles will be obtained from the group Kreßler (TP 3). Investigations on the lyotropic behaviour and their aggregation in water will be carried out first. Thereby influence of the chemical structure of the side chains as well as the block sequence will be studied followed by investigations of the surface activity of the compounds at the air-water interface. Lipid model membranes and lipid monolayers will serve as templates for the binding of the polyphiles and resulting structure formation at the interface. The binding strength is due to hydrophobic and hydrophilic interactions with the membranes and will be varied by changing the chemical structure of the polyphiles. The basic methods for studying the aggregation behaviour are light scattering methods, transmission electron microscopy, isothermal titration calorimetry (ITC) for the determination of the critical micellar concentration (cmc), as well as monolayer methods (epi-fluorescence-spectroscopy, infrared-reflection-absorptionspectroscopy (IRRAS) Brewster-angle-microscopy). Studies on the interaction of polyphiles with lipid bilayers and lipid monolayers will be carried out in a parallel fashion. Goal of studies with the low molecular weight polyphiles is to modify the interactions of these molecules and the resulting structure formation and segregation in or at the membranes by changes in the lateral chain structure. For polymeric polyphiles possible chiral recognition between polymers and chiral lipids plays an important addional role. For these studies thermal methods such as ITC and DSC will be used as well as spectroscopic methods such as FT-IR-Spectroscopy on solid supported membranes (FT-IR-ATR) and FT-IRRAS on lipid monolayers. In cooperation with the group Bacia (TP 6) CLSM studies on GUVs will be performed coupled with dye efflux measurements. Similar dye release experiments with small vesicles will also be carried out to elucidate the integrity of the permeation barrier of the membrane after incorporation of polyphiles. In close cooperation with the group Saalwächter (TP 5) additional NMR-experiments will be carried out with the same systems.

本项目的目标是研究和阐明水溶液中低分子和聚合物多亲分子与脂质模型膜和脂单分子膜的相互作用。低分子量多聚体将具有类似T-和X的结构，并将在C.Tschierske(TP 2)基团中合成，而聚合物多聚体将从Kreüler(TP 3)基团中得到。首先将对它们在水中的溶致行为和聚集进行研究。因此，将研究侧链的化学结构以及嵌段顺序的影响，随后将研究化合物在空气-水界面的表面活性。脂质模型膜和脂单分子层将作为模板，用于多聚体的结合和所产生的界面结构形成。结合强度是由于与膜的疏水和亲水相互作用，并将通过改变多聚体的化学结构来改变。研究聚集行为的基本方法有光散射法、透射电子显微镜、测定临界胶束浓度(CMC)的等温滴定热法(ITC)以及单层法(EPI荧光光谱、红外反射吸收光谱(IRRAS)布鲁斯特角显微镜)。多聚体与脂类双层和脂类单层的相互作用的研究将以平行的方式进行。研究低分子量多聚体的目的是通过改变侧链结构来改变这些分子之间的相互作用，以及由此产生的结构形成和膜内或膜上的分离。对于多亲聚合物，聚合物与手性脂类之间可能的手性识别起着重要的辅助作用。在这些研究中，将使用热方法，如ITC和DSC，以及光谱方法，如固体支撑膜上的FT-IR-光谱(FT-IR-ATR)和脂单分子膜上的FT-IRRAS。与BAKIA(TP 6)小组合作，将结合染料外流测量对GUV进行CLSM研究。还将进行类似的小囊泡染料释放实验，以阐明在加入多聚体后膜的渗透屏障的完整性。在与Saalwächter(TP 5)小组的密切合作下，将使用相同的系统进行额外的核磁共振实验。