The influence of gamete phenotype, gamete genotype and behavior on reproductive success in broadcast spawning invertebrates

配子表型、配子基因型和行为对广播产卵无脊椎动物繁殖成功的影响

• 批准号: 0241678
• 负责人: Don Levitan
• 金额: --
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0241678&HistoricalAwards=false
• 关键词: influence; gamete; phenotype; genotype; behavior

Selection via reproductive success can have a major influence on the evolution of traits and patterns of gender dimorphism. Most research has focused on scenarios in which selection is more intense for one gender than the other. Generally, males are thought to compete for mates while females choose among potential mates, resulting in high variance in male reproductive success and low variance in female reproductive success. However, previous work has shown that at some population densities, variance in both male and female success can be high in broadcast spawning marine invertebrates. This project will address the consequences of these patterns for fecundity selection in this primitive mating strategy. Three species of sea urchins will be used to address how spawning behavior, population density, and gamete phenotype and genotype influence reproductive success. Microsatellite markers will be used to establish maternity and paternity of larvae generated from laboratory and field experiments. The relative contributions of gamete recognition proteins and gamete morphology to reproductive success will be assessed, and the consequences of gender differences in spawning behavior in the presence and absence of gamete competition will be examined. This study will provide insight into the resolution of fecundity selection and gender conflict in the sea. Broader impacts will include the training of numerous undergraduate, recent graduate and graduate student assistants. The research program will be used as a mechanism to teach science in undergraduate and graduate courses taught at both at Florida State University and at the Bamfield Marine Sciences Center.

通过繁殖成功进行的选择可以对性状的进化和性别二态化模式产生重大影响。大多数研究都集中在一种性别的选择比另一种性别更强烈的情况下。一般来说，男性被认为是竞争配偶，而女性在潜在的配偶中选择，导致男性繁殖成功率的差异很大，而女性繁殖成功率的差异很小。然而，先前的研究表明，在一定的种群密度下，在广播产卵的海洋无脊椎动物中，雄性和雌性成功的差异可能很大。这个项目将解决在这种原始交配策略中这些模式对繁殖力选择的影响。将使用三种海胆来解决产卵行为，种群密度，配子表型和基因型如何影响生殖成功。微卫星标记将用于确定实验室和田间试验产生的幼虫的母系和父系。将评估配子识别蛋白和配子形态对繁殖成功的相对贡献，并研究在存在和不存在配子竞争的情况下产卵行为的性别差异的后果。本研究将对海洋中繁殖力选择和性别冲突的解决提供深入的见解。更广泛的影响将包括培养大量本科生、应届毕业生和研究生助理。该研究项目将作为一种机制，在佛罗里达州立大学和班菲尔德海洋科学中心的本科和研究生课程中教授科学。