An evolutionary and proteomic search for human gamete recognition proteins that mediate zona pellucida dissolution
介导透明带溶解的人类配子识别蛋白的进化和蛋白质组学研究
基本信息
- 批准号:10370974
- 负责人:
- 金额:$ 22.93万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-09-22 至 2023-08-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAddressAffinityBindingBiochemicalBioinformaticsBiological AssayBiologyCharacteristicsChemotaxisClassificationCodon NucleotidesComplementary DNADataDatabasesDetectionDistantEgg ProteinsEventEvolutionExploratory/Developmental Grant for Diagnostic Cancer ImagingFemaleFertilizationFrequenciesFutureGenesGeneticGenomicsGerm CellsGoalsGuanine + Cytosine CompositionHomologous GeneHomologous ProteinHumanHuman GenomeKnock-outLengthMass Spectrum AnalysisMeasuresMediatingMolecularMorphologyMusN DomainOocytesPerformanceProtein Structure InitiativeProteinsProteomeProteomicsRNA SplicingReproductionResearchResourcesSpecificitySperm CapacitationStructureSurface Plasmon ResonanceTechnologyTestingTestisTranscriptVariantZona Pellucidaabalonealgorithm trainingbaseeggenv Gene Productsexperienceextracellulargenetic approachgenomic dataimprovedinnovationlysinmalemammalian genomemolecular phenotypenanoporeprediction algorithmpredictive testreproductivesperm cellsperm proteinthree dimensional structuretranscriptometranscriptome sequencingtranscriptomics
项目摘要
Project Summary
The identification of interacting male-female reproductive proteins is imperative for a molecular understanding
of fertilization. The goal of this R21 proposal is to identify a set of human gamete recognition proteins using
genomic and proteomic approaches, and then perform evolutionary guided computational bioinformatic
searches for a homolog of abalone sperm lysin. It is known from three-dimensional protein crystallographic
studies of the major proteins from the mammalian Zona Pellucida (ZP) and abalone Vitelline Envelope (VE)
that they contain homologous ZP-N domains. Given the molecular homology of ZP-N domains and overall
biochemical similarity of the egg coats, an obvious question to address is whether sperm use similar
mechanisms (or homologous proteins) to generate a path through the egg coat. The dissolution of the abalone
egg VE by the sperm protein lysin has been extensively characterized. However, it remains unknown how
mammalian sperm penetrate egg coats, and no lysin homolog is currently identifiable in any mammalian
genome. Our proposal address this question by (1) using long-read sequencing to generate deep coverage of
full-length non-chimeric cDNAs for use in sperm proteomics, (2) using these resources as a database to
perform detailed bioinformatic analyses aimed at generating a database of putative human gamete recognition
proteins and identification of a human homolog of abalone sperm lysin and (3) functional tests of predicted
protein's ability to disrupt the mouse ZP. If we identify a human sperm lysin, it will immediately open up new
avenues of research into mammalian gamete recognition and how the human sperm passes the ZP. If we are
unsuccessful at identifying a human lysin, we will have generated a valuable genomic and proteomic resource
database for future studies of human sperm biology.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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WILLIE J SWANSON其他文献
WILLIE J SWANSON的其他文献
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{{ truncateString('WILLIE J SWANSON', 18)}}的其他基金
An evolutionary and proteomic search for human gamete recognition proteins that mediate zona pellucida dissolution
介导透明带溶解的人类配子识别蛋白的进化和蛋白质组学研究
- 批准号:
10493286 - 财政年份:2021
- 资助金额:
$ 22.93万 - 项目类别:
Detailed functional analysis of gamete recognition protein interactions
配子识别蛋白相互作用的详细功能分析
- 批准号:
9086388 - 财政年份:2014
- 资助金额:
$ 22.93万 - 项目类别:
Detailed functional analysis of gamete recognition protein interactions
配子识别蛋白相互作用的详细功能分析
- 批准号:
8877577 - 财政年份:2014
- 资助金额:
$ 22.93万 - 项目类别:
Diversity Action Plan: UW GenOM Project
多样性行动计划:华盛顿大学 GenOM 项目
- 批准号:
9134763 - 财政年份:2013
- 资助金额:
$ 22.93万 - 项目类别:
Diversity Action Plan: UW GenOM Project
多样性行动计划:华盛顿大学 GenOM 项目
- 批准号:
8474236 - 财政年份:2013
- 资助金额:
$ 22.93万 - 项目类别:
Diversity Action Plan: UW GenOM Project
多样性行动计划:华盛顿大学 GenOM 项目
- 批准号:
8642204 - 财政年份:2013
- 资助金额:
$ 22.93万 - 项目类别:
Diversity Action Plan: UW GenOM Project
多样性行动计划:华盛顿大学 GenOM 项目
- 批准号:
9356553 - 财政年份:2013
- 资助金额:
$ 22.93万 - 项目类别:
Diversity Action Plan: UW GenOM Project
多样性行动计划:华盛顿大学 GenOM 项目
- 批准号:
8727090 - 财政年份:2013
- 资助金额:
$ 22.93万 - 项目类别:
PROTEOMICS REVEALS RAPIDLY EVOLVING POLLEN TUBE PROTEINS
蛋白质组学揭示快速进化的花粉管蛋白质
- 批准号:
8365839 - 财政年份:2011
- 资助金额:
$ 22.93万 - 项目类别:
FUNCTIONAL DIVERSIFICATION AND EVOLUTION OF ANTIFREEZE PROTEINS
抗冻蛋白的功能多样化和进化
- 批准号:
8365846 - 财政年份:2011
- 资助金额:
$ 22.93万 - 项目类别:
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