Integrated Multistage Iso-electric Focusing on a PDMS Microchip.

建议标题：集成多级等电聚焦于PDMS微芯片建议编号：CTS-0300802首席研究员：Prashanta dutaInstitution：华盛顿州大学拟议研究的目标是在结合紫外线(UV)检测系统的聚二甲基硅氧烷(PDMS)芯片平台上实现多级等电聚焦(IEF)。建议的IEF系统将用于更快、更高分辨率地分离蛋白质。分段允许将分离分布在多通道阵列上。如果次级阶段能够并行运行，预计这一进程将显著加快。微通道将由PDMS制成，并在光学工作台上使用UV检测系统进行测试。将制造越来越复杂的几何形状的通道，以便可以顺序测试阶段所需的基本元件，并将其组合到先进的芯片设计中。IEF系统的一维模型将用于模拟沟道转弯、T形三通和交叉的影响，以便在制造这些芯片之前对系统进行优化。就更广泛的影响而言，这项工作可能会影响蛋白质组学领域，该领域有可能建立基因表达和疾病病理学之间的联系。研究生和本科生都将参与这个多学科项目。

AbstractProposal Title: Integrated Multistage Iso-electric Focusing on a PDMS MicrochipProposal Number: CTS-0300802Principal Investigator: Prashanta DuttaInstitution: Washington State UniversityThe objective of the proposed research is to implement multi-stage isoelectric focusing (IEF) in a poly-dimethlysiloxane (PDMS) chip platform coupled with an ultraviolet (UV) light detection system. The proposed IEF system will be used to fractionate proteins more quickly and with greater resolution. Staging allows the separation to be distributed across a multi-channel array. If the secondary stages can be run in parallel, it is expected that the process can be accelerated significantly. Microchannels will be fabricated from PDMS and tested on an optical bench using a UV detection system. Channels of increasingly complex geometry will be fabricated so that the fundamental elements required for staging can be sequentially tested and combined into an advanced chip design. One-dimensional models of the IEF system will be used to simulate the effects of channel turns, tees, and crosses so that the system can be optimized before these chips are fabricated. In terms of the broader impacts, this work may impact the field of proteomics, which has the potential to establish the link between gene expression and disease pathology. Both graduate and undergraduate students will be involved in this multidisciplinary project.