An Isothermal Titration Calorimeter to Study Thermodynamics of Molecular Recognition and Function

用于研究分子识别和功能热力学的等温滴定量热仪

• 批准号: 0301673
• 负责人: Yuri Sykulev
• 金额: $ 5.57万
• 依托单位: Thomas Jefferson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0301673&HistoricalAwards=false
• 关键词: Isothermal; Titration; Calorimeter; Study; Thermodynamics

This award supports the purchase of an Isothermal Titration Calorimeter (ITC) to be used for experimental thermodynamic analyses of biomolecular recognition and function. Calorimetric studies permit characterization of enzymatic reactions and, as well, study of non-covalent interactions between proteins and various other molecules, both large and small. The instrument will be placed in the University's core facility for protein studies where it will be available to faculty from throughout the institution. Initially, five investigators and thirty to fifty trainees in their laboratories are expected to be the major users. Planned studies that will use the instrument include the experimental identification of substrates of nitrilase-related enzymes; analysis of dual specificity tRNA synthetases; characterization of protein/DNA and protein/protein interactions important for avidity; measurement of the binding of small cyclic peptides and their cellular targets; and evaluation of the thermodynamics interaction of T-cell receptors and their natural signaling ligands. In addition to its use in research, the instrument will be used to support a curriculum change in the University's graduate biophysics course that will provide a new emphasis on calorimetric approaches to the study of molecular interactions.

该合同支持购买等温滴定量热计（ITC），用于生物分子识别和功能的实验热力学分析。量热法研究可以表征酶促反应，也可以研究蛋白质和其他各种大小分子之间的非共价相互作用。该仪器将放置在该大学蛋白质研究的核心设施中，供整个机构的教师使用。最初，预计5名研究人员和他们实验室的30至50名受训人员将是主要用户。将使用该仪器的计划研究包括对硝化酶相关酶底物的实验鉴定；双特异性tRNA合成酶分析；蛋白质/DNA和蛋白质/蛋白质相互作用对贪婪性的重要表征；小环肽与细胞靶点结合的测定以及t细胞受体及其天然信号配体的热力学相互作用的评价。除了用于研究之外，该仪器还将用于支持该大学研究生生物物理学课程的课程改革，该课程将重点放在研究分子相互作用的量热方法上。