High-Throughput Gene Expression and Proteomics Instrumentation for Functional Genomics

用于功能基因组学的高通量基因表达和蛋白质组学仪器

• 批准号: 0301761
• 负责人: Vivek Kapur
• 金额: $ 31.92万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0301761&HistoricalAwards=false
• 关键词: Throughput; Gene; Expression; Proteomics; Instrumentation

This award supports the purchase of three instruments to be used together for functional genomics studies, a new area of biological research focused on discovery and characterization of genes, gene products, and their associated functions. Such studies, which are now well underway for many microbes, plants, and animals, typically require access to high-throughput instrumentation for detailed analysis of DNA, RNA and protein. The instruments to be purchased will be used for high-throughput real-time polymerase chain reaction (PCR) analyses of gene expression, measurement of nucleic acids and proteins using state of the art lab-on-a-chip technology, and identification of new and known proteins through coupled liquid chromatography - mass spectrometry. Research programs expected to take advantage of the equipment include studies of plant-microbe interactions in several plants, epigenetic regulation of gene expression in maize, gene regulation in oats, evolutionary genomics of plant development, and effect of sugars on plant metabolic pathways. The instruments will be located in a central facility that insures instrument access for over 100 research groups, including faculty and students.

这项奖励支持购买三种仪器一起用于功能基因组学研究，这是生物学研究的一个新领域，专注于基因、基因产品及其相关功能的发现和表征。这类研究目前正在对许多微生物、植物和动物进行，通常需要使用高通量仪器对DNA、RNA和蛋白质进行详细分析。购买的仪器将用于高通量实时聚合酶链式反应(PCR)基因表达分析，使用最先进的芯片实验室技术测量核酸和蛋白质，并通过液-质联用识别新的和已知的蛋白质。预计将利用这些设备的研究项目包括几种植物中植物-微生物相互作用的研究，玉米基因表达的表观遗传调控，燕麦基因调控，植物发育的进化基因组学，以及糖对植物代谢途径的影响。这些仪器将放置在一个中央设施中，确保包括教职员工和学生在内的100多个研究小组可以使用仪器。