High-Throughput Gene Expression and Proteomics Instrumentation for Functional Genomics

该奖项支持购买三种仪器一起用于功能基因组学研究，这是一个新的生物学研究领域，专注于发现和表征基因，基因产物及其相关功能。 这些研究目前正在对许多微生物、植物和动物进行研究，通常需要使用高通量仪器来详细分析DNA、RNA和蛋白质。 采购的仪器将用于基因表达的高通量实时聚合酶链反应分析、使用最先进的芯片实验室技术测量核酸和蛋白质，以及通过液相色谱-质谱联用鉴定新的和已知的蛋白质。 预计利用该设备的研究计划包括几种植物中植物-微生物相互作用的研究，玉米基因表达的表观遗传调控，燕麦的基因调控，植物发育的进化基因组学以及糖对植物代谢途径的影响。这些仪器将位于一个中央设施，确保100多个研究小组，包括教师和学生的仪器访问。

This award supports the purchase of three instruments to be used together for functional genomics studies, a new area of biological research focused on discovery and characterization of genes, gene products, and their associated functions. Such studies, which are now well underway for many microbes, plants, and animals, typically require access to high-throughput instrumentation for detailed analysis of DNA, RNA and protein. The instruments to be purchased will be used for high-throughput real-time polymerase chain reaction (PCR) analyses of gene expression, measurement of nucleic acids and proteins using state of the art lab-on-a-chip technology, and identification of new and known proteins through coupled liquid chromatography - mass spectrometry. Research programs expected to take advantage of the equipment include studies of plant-microbe interactions in several plants, epigenetic regulation of gene expression in maize, gene regulation in oats, evolutionary genomics of plant development, and effect of sugars on plant metabolic pathways. The instruments will be located in a central facility that insures instrument access for over 100 research groups, including faculty and students.