Instrumentation Systems for Microbial "Omics": Discovery and Quantitation of Biomolecules and Natural Substrates

微生物“组学”仪器系统：生物分子和天然底物的发现和定量

• 批准号: 0304606
• 负责人: Patricia Sobecky
• 金额: $ 25.41万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0304606&HistoricalAwards=false
• 关键词: Instrumentation; Systems; Microbial; Omics; Discovery

This award provides support for the purchase of: (1) real-time PCR instrumentation, (2) a micro-array spotter and scanner system, (3) lab-scale fermentation equipment, (4) capillary electrophoresis equipment and, (5) high-pressure biomolecule separation equipment. The goals of the research programs that will utilize the instrumentation are to characterize biomolecules (proteins, plasmids), natural substrates (metal oxides), and microbial populations from diverse aquatic and terrestrial environments. The researchers will also investigate the role of chemical speciation on microbial processes by detecting substrates (i.e., metal complexes, organic metabolites) that may regulate the mechanisms of substrate utilization, biomolecule production, and bacterial growth. The users of the instrumentation represent faculty from two different Georgia Institute of Technology colleges (College of Science and the College of Engineering) from the School of Biology, and Departments of Civil and Environmental Engineering and Earth and Atmospheric Sciences. The anticipated interactions between the various user groups will provide the basis for a new generation of scientists with the ability to communicate effectively across research disciplines. In addition, this award will allow the PIs to train and mentor a diverse group of students and researchers including those traditionally underrepresented in science.

该奖项为以下采购提供支持：（1）实时PCR仪器，（2）微阵列点样仪和扫描仪系统，（3）实验室规模的发酵设备，（4）毛细管电泳设备和（5）高压生物分子分离设备。将利用仪器的研究计划的目标是表征生物分子（蛋白质，质粒），天然底物（金属氧化物），以及来自不同水生和陆地环境的微生物种群。 研究人员还将通过检测底物（即，金属络合物、有机代谢物），其可以调节底物利用、生物分子产生和细菌生长的机制。 仪器的用户代表来自两个不同的格鲁吉亚理工学院（理学院和工程学院）从生物学院，土木和环境工程系和地球与大气科学系的教师。 不同用户群体之间的预期互动将为新一代科学家提供跨研究学科有效沟通的基础。 此外，该奖项将允许PI培训和指导不同群体的学生和研究人员，包括那些传统上在科学领域代表性不足的学生和研究人员。